Re-decontamination of Liquid Mycobacterial Cultures: Additional Yield in the Era of Xpert MTB/RIF Ultra in Cape Town, South Africa
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Abstract
A retrospective review of liquid mycobacterial cultures was performed at a laboratory in South Africa from 01 January 2018 to 31 December 2018 to assess the increased yield in detecting complex following sample re-decontamination. Only 9 of 99 (9%) re-decontaminated samples were culture positive for complex. Xpert MTB/RIF Ultra, concurrently performed on 7 of the 9 samples, detected complex in all but 1 sample. Re-decontamination of non-sterile samples did not increase the complex yield enough to offset the financial costs and additional labour in a laboratory that utilises the Xpert MTB/RIF Ultra system as a first-line diagnostic modality.
References
1.
Lewinsohn D, Leonard M, LoBue P, Cohn D, Daley C, Desmond E
. Official American Thoracic Society/Infectious Diseases Society of America/Centers for Disease Control and Prevention Clinical Practice Guidelines: Diagnosis of Tuberculosis in Adults and Children. Clin Infect Dis. 2016; 64(2):e1-e33.
DOI: 10.1093/cid/ciw694.
View
2.
Chakravorty S, Simmons A, Rowneki M, Parmar H, Cao Y, Ryan J
. The New Xpert MTB/RIF Ultra: Improving Detection of and Resistance to Rifampin in an Assay Suitable for Point-of-Care Testing. mBio. 2017; 8(4).
PMC: 5574709.
DOI: 10.1128/mBio.00812-17.
View
3.
Dorman S, Schumacher S, Alland D, Nabeta P, Armstrong D, King B
. Xpert MTB/RIF Ultra for detection of Mycobacterium tuberculosis and rifampicin resistance: a prospective multicentre diagnostic accuracy study. Lancet Infect Dis. 2017; 18(1):76-84.
PMC: 6168783.
DOI: 10.1016/S1473-3099(17)30691-6.
View
4.
Mtafya B, Sabiiti W, Sabi I, John J, Sichone E, Ntinginya N
. Molecular Bacterial Load Assay Concurs with Culture on NaOH-Induced Loss of Mycobacterium tuberculosis Viability. J Clin Microbiol. 2019; 57(7).
PMC: 6595441.
DOI: 10.1128/JCM.01992-18.
View