Identification and Characterization of a Novel Gene Aokap1 Involved in Growth and Kojic Acid Synthesis in Aspergillus Oryzae

Overview

Journal Arch Microbiol

Specialty Microbiology

Date 2021 Dec 24

PMID 34950983

Citations 6

Authors

Yuzhen Li

Huanxin Zhang

Ziming Chen

Junxia Fan

Tianming Chen

Bin Zeng

Zhe Zhang

Affiliations

Soon will be listed here.

Abstract

The elucidation of the mechanism for the growth and secondary metabolites in Aspergillus oryzae is important for industrial production and utilization. Here, we found that the expression of a novel gene Aokap1 was induced during the growth of A. oryzae. Sequence analysis revealed that AoKap1 has four transmembrane regions and is conserved in Aspergillus species. Disruption of Aokap1 caused the inhibition in mycelium growth and conidia formation, corresponding with reduced expression of brlA and abaA. Furthermore, deletion of Aokap1 resulted in elevated production of kojic acid, and the expression of kojA, kojR and kojT was up-regulated in Aokap1-disrupted strain. Meanwhile, overexpression of kojR resulted in the decreased expression of Aokap1, suggesting that disruption of Aokap1 increased kojic acid production by affecting the expression of kojA, kojR and kojT. The discovery of Aokap1 provides a new target for genetic modification of the growth and kojic acid production in A. oryzae.

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References

Abe K, Gomi K, Hasegawa F, Machida M . Impact of Aspergillus oryzae genomics on industrial production of metabolites. Mycopathologia. 2006; 162(3):143-53. DOI: 10.1007/s11046-006-0049-2. View

Adams T, Boylan M, Timberlake W . brlA is necessary and sufficient to direct conidiophore development in Aspergillus nidulans. Cell. 1988; 54(3):353-62. DOI: 10.1016/0092-8674(88)90198-5. View

Adams T, Wieser J, Yu J . Asexual sporulation in Aspergillus nidulans. Microbiol Mol Biol Rev. 1998; 62(1):35-54. PMC: 98905. DOI: 10.1128/MMBR.62.1.35-54.1998. View

Andrianopoulos A, Timberlake W . The Aspergillus nidulans abaA gene encodes a transcriptional activator that acts as a genetic switch to control development. Mol Cell Biol. 1994; 14(4):2503-15. PMC: 358618. DOI: 10.1128/mcb.14.4.2503-2515.1994. View

Arakawa G, Kudo H, Yanase A, Eguchi Y, Kodama H, Ogawa M . A unique Zn(II)-Cys-type protein, KpeA, is involved in secondary metabolism and conidiation in Aspergillus oryzae. Fungal Genet Biol. 2019; 127:35-44. DOI: 10.1016/j.fgb.2019.02.004. View

Baltussen T, Zoll J, Verweij P, Melchers W . Molecular Mechanisms of Conidial Germination in spp. Microbiol Mol Biol Rev. 2019; 84(1). PMC: 6903801. DOI: 10.1128/MMBR.00049-19. View

Chen X, Zhou J, Ding Q, Luo Q, Liu L . Morphology engineering of Aspergillus oryzae for l-malate production. Biotechnol Bioeng. 2019; 116(10):2662-2673. DOI: 10.1002/bit.27089. View

Etxebeste O, Espeso E . Aspergillus nidulans in the post-genomic era: a top-model filamentous fungus for the study of signaling and homeostasis mechanisms. Int Microbiol. 2019; 23(1):5-22. DOI: 10.1007/s10123-019-00064-6. View

Fan J, Zhang Z, Long C, He B, Hu Z, Jiang C . Identification and functional characterization of glycerol dehydrogenase reveal the role in kojic acid synthesis in Aspergillus oryzae. World J Microbiol Biotechnol. 2020; 36(9):136. DOI: 10.1007/s11274-020-02912-4. View

10.

Gomi K . Regulatory mechanisms for amylolytic gene expression in the koji mold . Biosci Biotechnol Biochem. 2019; 83(8):1385-1401. DOI: 10.1080/09168451.2019.1625265. View

11.

Ichishima E . Development of enzyme technology for Aspergillus oryzae, A. sojae, and A. luchuensis, the national microorganisms of Japan. Biosci Biotechnol Biochem. 2016; 80(9):1681-92. DOI: 10.1080/09168451.2016.1177445. View

12.

Jeennor S, Anantayanon J, Panchanawaporn S, Chutrakul C, Laoteng K . Morphologically engineered strain of Aspergillus oryzae as a cell chassis for production development of functional lipids. Gene. 2019; 718:144073. DOI: 10.1016/j.gene.2019.144073. View

13.

Kobayashi T, Abe K, Asai K, Gomi K, Juvvadi P, Kato M . Genomics of Aspergillus oryzae. Biosci Biotechnol Biochem. 2007; 71(3):646-70. DOI: 10.1271/bbb.60550. View

14.

Krijgsheld P, Bleichrodt R, van Veluw G, Wang F, Muller W, Dijksterhuis J . Development in Aspergillus. Stud Mycol. 2013; 74(1):1-29. PMC: 3563288. DOI: 10.3114/sim0006. View

15.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

16.

Machida M, Asai K, Sano M, Tanaka T, Kumagai T, Terai G . Genome sequencing and analysis of Aspergillus oryzae. Nature. 2005; 438(7071):1157-61. DOI: 10.1038/nature04300. View

17.

Machida M, Yamada O, Gomi K . Genomics of Aspergillus oryzae: learning from the history of Koji mold and exploration of its future. DNA Res. 2008; 15(4):173-83. PMC: 2575883. DOI: 10.1093/dnares/dsn020. View

18.

Marshall M, Timberlake W . Aspergillus nidulans wetA activates spore-specific gene expression. Mol Cell Biol. 1991; 11(1):55-62. PMC: 359587. DOI: 10.1128/mcb.11.1.55-62.1991. View

19.

Marui J, Ohashi-Kunihiro S, Ando T, Nishimura M, Koike H, Machida M . Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering. J Biosci Bioeng. 2010; 110(1):8-11. DOI: 10.1016/j.jbiosc.2010.01.001. View

20.

Marui J, Yamane N, Ohashi-Kunihiro S, Ando T, Terabayashi Y, Sano M . Kojic acid biosynthesis in Aspergillus oryzae is regulated by a Zn(II)(2)Cys(6) transcriptional activator and induced by kojic acid at the transcriptional level. J Biosci Bioeng. 2011; 112(1):40-3. DOI: 10.1016/j.jbiosc.2011.03.010. View