Activin A Promotes Osteoblastic Differentiation of Human Preosteoblasts Through the ALK1-Smad1/5/9 Pathway

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Dec 24

PMID 34948289

Citations 3

Authors

Hideki Sugii

Mhd Safwan Albougha

Orie Adachi

Hiroka Tomita

Atsushi Tomokiyo

Sayuri Hamano

Daigaku Hasegawa

Shinichiro Yoshida

Tomohiro Itoyama

Hidefumi Maeda

Affiliations

Soon will be listed here.

Abstract

Activin A, a member of transforming growth factor-β superfamily, is involved in the regulation of cellular differentiation and promotes tissue healing. Previously, we reported that expression of activin A was upregulated around the damaged periodontal tissue including periodontal ligament (PDL) tissue and alveolar bone, and activin A promoted PDL-related gene expression of human PDL cells (HPDLCs). However, little is known about the biological function of activin A in alveolar bone. Thus, this study analyzed activin A-induced biological functions in preosteoblasts (Saos2 cells). Activin A promoted osteoblastic differentiation of Saos2 cells. Activin receptor-like kinase (ALK) 1, an activin type I receptor, was more strongly expressed in Saos2 cells than in HPDLCs, and knockdown of ALK1 inhibited activin A-induced osteoblastic differentiation of Saos2 cells. Expression of ALK1 was upregulated in alveolar bone around damaged periodontal tissue when compared with a nondamaged site. Furthermore, activin A promoted phosphorylation of Smad1/5/9 during osteoblastic differentiation of Saos2 cells and knockdown of ALK1 inhibited activin A-induced phosphorylation of Smad1/5/9 in Saos2 cells. Collectively, these findings suggest that activin A promotes osteoblastic differentiation of preosteoblasts through the ALK1-Smad1/5/9 pathway and could be used as a therapeutic product for the healing of alveolar bone as well as PDL tissue.

Citing Articles

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The TGF-β/UCHL5/Smad2 Axis Contributes to the Pathogenesis of Placenta Accreta.

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Decorin Promotes Osteoblastic Differentiation of Human Periodontal Ligament Stem Cells.

Adachi O, Sugii H, Itoyama T, Fujino S, Kaneko H, Tomokiyo A Molecules. 2022; 27(23).

PMID: 36500314 PMC: 9739490. DOI: 10.3390/molecules27238224.

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