Why Is the Correct Selection of Strains Important? The Case of Wheat Endophytic Strains of and

Overview

Journal J Fungi (Basel)

Date 2021 Dec 24

PMID 34947069

Citations 11

Authors

Alberto Pedrero-Mendez

H Camilo Insuasti

Theodora Neagu

Maria Illescas

M Belen Rubio

Enrique Monte

Rosa Hermosa

Affiliations

Soon will be listed here.

Abstract

The search for endophytic fungi in the roots of healthy wheat plants from a non-irrigation field trial allowed us to select 4 out of a total of 54 cultivable isolates belonging to the genus , identified as T136 and T139, T137, and T138. In vitro assays against the phytopathogenic fungus showed that the strains had the highest biocontrol potential and that T136 exhibited the highest cellulase and chitinase activities. Production patterns of eight phytohormones varied among the strains. All four, when applied alone or in combination, colonized roots of other wheat cultivars and promoted seed germination, tillering, and plant growth under optimal irrigation conditions in the greenhouse. Apart from T136, the endophytic strains showed plant protection capacity against drought as they activated the antioxidant enzyme machinery of the wheat plants. However, T137 gave the best plant size and spike weight performance in water-stressed plants at the end of the crop. This trait correlated with significantly increased production of indole acetic acid and abscisic acid and increased 1-aminocyclopropane-1-carboxylic acid deaminase activity by T137. This study shows the potential of endophytes and that their success in agricultural systems requires careful selection of suitable strains.

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References

Mastouri F, Bjorkman T, Harman G . Trichoderma harzianum enhances antioxidant defense of tomato seedlings and resistance to water deficit. Mol Plant Microbe Interact. 2012; 25(9):1264-71. DOI: 10.1094/MPMI-09-11-0240. View

Zhang S, Gan Y, Xu B . Mechanisms of the IAA and ACC-deaminase producing strain of Trichoderma longibrachiatum T6 in enhancing wheat seedling tolerance to NaCl stress. BMC Plant Biol. 2019; 19(1):22. PMC: 6330461. DOI: 10.1186/s12870-018-1618-5. View

Druzhinina I, Kubicek C . Species concepts and biodiversity in Trichoderma and Hypocrea: from aggregate species to species clusters?. J Zhejiang Univ Sci B. 2005; 6(2):100-12. PMC: 1389624. DOI: 10.1631/jzus.2005.B0100. View

Zhu J . Salt and drought stress signal transduction in plants. Annu Rev Plant Biol. 2002; 53:247-73. PMC: 3128348. DOI: 10.1146/annurev.arplant.53.091401.143329. View

Seo M, Jikumaru Y, Kamiya Y . Profiling of hormones and related metabolites in seed dormancy and germination studies. Methods Mol Biol. 2011; 773:99-111. DOI: 10.1007/978-1-61779-231-1_7. View

Chaverri P, Branco-Rocha F, Jaklitsch W, Gazis R, Degenkolb T, Samuels G . Systematics of the Trichoderma harzianum species complex and the re-identification of commercial biocontrol strains. Mycologia. 2015; 107(3):558-590. PMC: 4885665. DOI: 10.3852/14-147. View

Moran-Diez M, Cardoza R, Gutierrez S, Monte E, Hermosa R . TvDim1 of Trichoderma virens is involved in redox-processes and confers resistance to oxidative stresses. Curr Genet. 2009; 56(1):63-73. DOI: 10.1007/s00294-009-0280-8. View

Bae H, Sicher R, Kim M, Kim S, Strem M, Melnick R . The beneficial endophyte Trichoderma hamatum isolate DIS 219b promotes growth and delays the onset of the drought response in Theobroma cacao. J Exp Bot. 2009; 60(11):3279-95. PMC: 2718224. DOI: 10.1093/jxb/erp165. View

Yedidia I , BENHAMOU , Chet I . Induction of defense responses in cucumber plants (Cucumis sativus L. ) By the biocontrol agent trichoderma harzianum . Appl Environ Microbiol. 1999; 65(3):1061-70. PMC: 91145. DOI: 10.1128/AEM.65.3.1061-1070.1999. View

10.

Nawaz A, Shahbaz M, Asadullah , Imran A, Marghoob M, Imtiaz M . Potential of Salt Tolerant PGPR in Growth and Yield Augmentation of Wheat ( L.) Under Saline Conditions. Front Microbiol. 2020; 11:2019. PMC: 7562815. DOI: 10.3389/fmicb.2020.02019. View

11.

Sanz L, Montero M, Grondona I, Vizcaino J, Llobell A, Hermosa R . Cell wall-degrading isoenzyme profiles of Trichoderma biocontrol strains show correlation with rDNA taxonomic species. Curr Genet. 2004; 46(5):277-86. DOI: 10.1007/s00294-004-0532-6. View

12.

Samolski I, Rincon A, Pinzon L, Viterbo A, Monte E . The qid74 gene from Trichoderma harzianum has a role in root architecture and plant biofertilization. Microbiology (Reading). 2011; 158(Pt 1):129-138. DOI: 10.1099/mic.0.053140-0. View

13.

Fleet G, Phaff H . Glucanases in Schizosaccharomyces. Isolation and properties of the cell wall-associated beta(1 leads to 3)-glucanases. J Biol Chem. 1974; 249(6):1717-28. View

14.

Schirmbock M, Lorito M, Wang Y, Hayes C, Scala F, Harman G . Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi. Appl Environ Microbiol. 1994; 60(12):4364-70. PMC: 201994. DOI: 10.1128/aem.60.12.4364-4370.1994. View

15.

Fontana D, de Paula S, Torres A, de Souza V, Pascholati S, Schmidt D . Endophytic Fungi: Biological Control and Induced Resistance to Phytopathogens and Abiotic Stresses. Pathogens. 2021; 10(5). PMC: 8151296. DOI: 10.3390/pathogens10050570. View

16.

Rubio M, Dominguez S, Monte E, Hermosa R . Comparative study of Trichoderma gene expression in interactions with tomato plants using high-density oligonucleotide microarrays. Microbiology (Reading). 2011; 158(Pt 1):119-128. DOI: 10.1099/mic.0.052118-0. View

17.

Perez E, Rubio M, Cardoza R, Gutierrez S, Bettiol W, Monte E . The importance of chorismate mutase in the biocontrol potential of Trichoderma parareesei. Front Microbiol. 2015; 6:1181. PMC: 4621298. DOI: 10.3389/fmicb.2015.01181. View

18.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

19.

Tseng Y, Rouina H, Groten K, Rajani P, Furch A, Reichelt M . An Endophytic Strain Promotes Growth of Its Hosts and Defends Against Pathogen Attack. Front Plant Sci. 2021; 11:573670. PMC: 7793846. DOI: 10.3389/fpls.2020.573670. View

20.

Jaklitsch W, Voglmayr H . Biodiversity of Trichoderma (Hypocreaceae) in Southern Europe and Macaronesia. Stud Mycol. 2016; 80:1-87. PMC: 4779795. DOI: 10.1016/j.simyco.2014.11.001. View