Transcriptome Analysis Reveals the Gene Expression Changes in the Silkworm () in Response to Hydrogen Sulfide Exposure

Overview

Journal Insects

Specialty Biology

Date 2021 Dec 23

PMID 34940198

Citations 4

Authors

Rui Zhang

Yu-Yao Cao

Juan Du

Kiran Thakur

Shun-Ming Tang

Fei Hu

Zhao-Jun Wei

Affiliations

Soon will be listed here.

Abstract

Hydrogen sulfide (HS) has been recognized for its beneficial influence on physiological alterations. The development (body weight) and economic characteristics (cocoon weight, cocoon shell ratio, and cocoon shell weight) of silkworms were increased after continuous 7.5 µM HS treatment. In the present study, gene expression changes in the fat body of silkworms at the 5th instar larvae in response to the HS were investigated through comparative transcriptome analysis. Moreover, the expression pattern of significant differentially expressed genes (DEGs) at the 5th instar larvae was confirmed by quantitative real-time PCR (qRT-PCR) after HS exposure. A total of 1200 (DEGs) was identified, of which 977 DEGs were up-regulated and 223 DEGs were down-regulated. Most of the DEGs were involved in the transport pathway, cellular community, carbohydrate metabolism, and immune-associated signal transduction. The up regulated genes under HS exposure were involved in endocytosis, glycolysis/gluconeogenesis, the citrate cycle (TCA cycle), and the synthesis of fibroin, while genes related to inflammation were down-regulated, indicating that HS could promote energy metabolism, the transport pathway, silk synthesis, and inhibit inflammation in the silkworm. In addition, the expression levels of these genes were increased or decreased in a time-dependent manner during the 5th instar larvae. These results provided insight into the effects of HS on silkworms at the transcriptional level and a substantial foundation for understanding HS function.

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