Citrobacter Rodentium(ϕStx2dact), a Murine Infection Model for Enterohemorrhagic Escherichia Coli

Overview

Journal Curr Opin Microbiol

Specialty Microbiology

Date 2021 Dec 20

PMID 34929548

Citations 6

Authors

Cheleste M Thorpe

Amanda R Pulsifer

Marcia S Osburne

Sivapriya Kailasan Vanaja

John M Leong

Affiliations

Soon will be listed here.

Abstract

The formation of attaching and effacing (A/E) lesions on intestinal epithelium, combined with Shiga toxin production, are hallmarks of enterohemorrhagic Escherichia coli (EHEC) infection that can lead to lethal hemolytic uremic syndrome. Although an animal infection model that fully recapitulates human disease remains elusive, mice orally infected with Citrobacter rodentium(ϕStx2dact), a natural murine pathogen lysogenized with an EHEC-derived Shiga toxin 2-producing bacteriophage, develop intestinal A/E lesions and toxin-dependent systemic disease. This model has facilitated investigation of how: (A) phage gene expression and prophage induction contribute to disease and are potentially triggered by antibiotic treatment; (B) virulence gene expression is altered by microbiota and the colonic metabolomic milieu; and (C) innate immune signaling is affected by Stx. Thus, the model provides a unique tool for accessing diverse aspects of EHEC pathogenesis.

Citing Articles

deposition of nanobodies by an engineered commensal microbe promotes survival in a mouse model of enterohemorrhagic .

Srivastava R, Gonzalez-Prieto C, Lynch J, Muscolo M, Lin C, Brown M PNAS Nexus. 2024; 3(9):pgae374.

PMID: 39262854 PMC: 11388102. DOI: 10.1093/pnasnexus/pgae374.

Enterohemorrhagic (EHEC) disrupts intestinal barrier integrity in translational canine stem cell-derived monolayers.

Nagao I, Kawasaki M, Goyama T, Kim H, Call D, Ambrosini Y Microbiol Spectr. 2024; 12(10):e0096124.

PMID: 39162490 PMC: 11448187. DOI: 10.1128/spectrum.00961-24.

deposition of nanobodies by an engineered commensal microbe promotes survival in a mouse model of enterohemorrhagic .

Srivastava R, Gonzalez-Prieto C, Lynch J, Muscolo M, Lin C, Brown M bioRxiv. 2024; .

PMID: 39131305 PMC: 11312530. DOI: 10.1101/2024.07.30.605899.

Loop mediated isothermal amplification as a molecular diagnostic assay: Application and evaluation for detection of Enterohaemorrhagic (O157:H7).

Yinur D, Moges B, Hassen A, Tessema T Pract Lab Med. 2023; 37:e00333.

PMID: 37693632 PMC: 10492192. DOI: 10.1016/j.plabm.2023.e00333.

Ribosomes: The New Role of Ribosomal Proteins as Natural Antimicrobials.

Hurtado-Rios J, Carrasco-Navarro U, Almanza-Perez J, Ponce-Alquicira E Int J Mol Sci. 2022; 23(16).

PMID: 36012387 PMC: 9409020. DOI: 10.3390/ijms23169123.

References

Wang J, Shao Y, Wang W, Li S, Xin N, Xie F . Caspase-11 deficiency impairs neutrophil recruitment and bacterial clearance in the early stage of pulmonary Klebsiella pneumoniae infection. Int J Med Microbiol. 2017; 307(8):490-496. DOI: 10.1016/j.ijmm.2017.09.012. View

Balasubramanian S, Osburne M, BrinJones H, Tai A, Leong J . Prophage induction, but not production of phage particles, is required for lethal disease in a microbiome-replete murine model of enterohemorrhagic E. coli infection. PLoS Pathog. 2019; 15(1):e1007494. PMC: 6328086. DOI: 10.1371/journal.ppat.1007494. View

Kayagaki N, Wong M, Stowe I, Ramani S, Gonzalez L, Akashi-Takamura S . Noncanonical inflammasome activation by intracellular LPS independent of TLR4. Science. 2013; 341(6151):1246-9. DOI: 10.1126/science.1240248. View

Rathinam V, Vanaja S, Waggoner L, Sokolovska A, Becker C, Stuart L . TRIF licenses caspase-11-dependent NLRP3 inflammasome activation by gram-negative bacteria. Cell. 2012; 150(3):606-19. PMC: 3660860. DOI: 10.1016/j.cell.2012.07.007. View

Flowers L, Bou Ghanem E, Leong J . Synchronous Disease Kinetics in a Murine Model for Enterohemorrhagic Infection Using Food-Borne Inoculation. Front Cell Infect Microbiol. 2016; 6:138. PMC: 5093121. DOI: 10.3389/fcimb.2016.00138. View

Tajiri H, Nishi J, Ushijima K, Shimizu T, Ishige T, Shimizu M . A role for fosfomycin treatment in children for prevention of haemolytic-uraemic syndrome accompanying Shiga toxin-producing Escherichia coli infection. Int J Antimicrob Agents. 2015; 46(5):586-9. DOI: 10.1016/j.ijantimicag.2015.08.006. View

Mohawk K, OBrien A . Mouse models of Escherichia coli O157:H7 infection and shiga toxin injection. J Biomed Biotechnol. 2011; 2011:258185. PMC: 3022220. DOI: 10.1155/2011/258185. View

Bekassy Z, Calderon Toledo C, Leoj G, Kristoffersson A, Leopold S, Perez M . Intestinal damage in enterohemorrhagic Escherichia coli infection. Pediatr Nephrol. 2010; 26(11):2059-71. DOI: 10.1007/s00467-010-1616-9. View

Menezes-Garcia Z, Kumar A, Zhu W, Winter S, Sperandio V . l-Arginine sensing regulates virulence gene expression and disease progression in enteric pathogens. Proc Natl Acad Sci U S A. 2020; 117(22):12387-12393. PMC: 7275701. DOI: 10.1073/pnas.1919683117. View

10.

Thomassin J, Giannakopoulou N, Zhu L, Gross J, Salmon K, Leclerc J . The CpxRA two-component system is essential for Citrobacter rodentium virulence. Infect Immun. 2015; 83(5):1919-28. PMC: 4399039. DOI: 10.1128/IAI.00194-15. View

11.

Richardson S, Karmali M, Becker L, Smith C . The histopathology of the hemolytic uremic syndrome associated with verocytotoxin-producing Escherichia coli infections. Hum Pathol. 1988; 19(9):1102-8. DOI: 10.1016/s0046-8177(88)80093-5. View

12.

Muhldorfer I, Hacker J, Keusch G, Acheson D, Tschape H, Kane A . Regulation of the Shiga-like toxin II operon in Escherichia coli. Infect Immun. 1996; 64(2):495-502. PMC: 173792. DOI: 10.1128/iai.64.2.495-502.1996. View

13.

Elbaz N, Socol Y, Katsowich N, Rosenshine I . Control of Type III Secretion System Effector/Chaperone Ratio Fosters Pathogen Adaptation to Host-Adherent Lifestyle. mBio. 2019; 10(5). PMC: 6751064. DOI: 10.1128/mBio.02074-19. View

14.

Curtis M, Hu Z, Klimko C, Narayanan S, DeBerardinis R, Sperandio V . The gut commensal Bacteroides thetaiotaomicron exacerbates enteric infection through modification of the metabolic landscape. Cell Host Microbe. 2014; 16(6):759-69. PMC: 4269104. DOI: 10.1016/j.chom.2014.11.005. View

15.

Obrig T, Karpman D . Shiga toxin pathogenesis: kidney complications and renal failure. Curr Top Microbiol Immunol. 2011; 357:105-36. PMC: 3779650. DOI: 10.1007/82_2011_172. View

16.

Osbelt L, Thiemann S, Smit N, Lesker T, Schroter M, Galvez E . Variations in microbiota composition of laboratory mice influence Citrobacter rodentium infection via variable short-chain fatty acid production. PLoS Pathog. 2020; 16(3):e1008448. PMC: 7141690. DOI: 10.1371/journal.ppat.1008448. View

17.

Kumar A, Russell R, Pifer R, Menezes-Garcia Z, Cuesta S, Narayanan S . The Serotonin Neurotransmitter Modulates Virulence of Enteric Pathogens. Cell Host Microbe. 2020; 28(1):41-53.e8. PMC: 7351610. DOI: 10.1016/j.chom.2020.05.004. View

18.

Raffa R, Raivio T . A third envelope stress signal transduction pathway in Escherichia coli. Mol Microbiol. 2002; 45(6):1599-611. DOI: 10.1046/j.1365-2958.2002.03112.x. View

19.

Havira M, Ta A, Kumari P, Wang C, Russo A, Ruan J . Shiga toxin suppresses noncanonical inflammasome responses to cytosolic LPS. Sci Immunol. 2020; 5(53). PMC: 7717664. DOI: 10.1126/sciimmunol.abc0217. View

20.

Psotka M, Obata F, Kolling G, Gross L, Saleem M, Satchell S . Shiga toxin 2 targets the murine renal collecting duct epithelium. Infect Immun. 2009; 77(3):959-69. PMC: 2643625. DOI: 10.1128/IAI.00679-08. View