Salidroside Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells Through the BMP Signaling Pathway

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2021 Dec 17

PMID 34917181

Citations 3

Authors

Xiaoling Wei

Jiayang Li

Hui Liu

Chenguang Niu

Dong Chen

Affiliations

Soon will be listed here.

Abstract

Regenerative endodontics, as an alternative approach, aims to regenerate dental pulp-like tissues and is garnering the attention of clinical dentists. This is due to its reported biological benefits for dental therapeutics. Stem cells and their microenvironment serve an important role in the process of pulp regeneration. Regulation of the stem cell microenvironment and the directed differentiation of stem cells is becoming a topic of intensive research. Salidroside (SAL) is extracted from the root of and it has been reported that SAL exerts antiaging, neuroprotective, hepatoprotective, cardioprotective and anticancer effects. However, the ability of SAL to regulate the osteo/odontogenic differentiation of hDPSCs remains to be elucidated. In the present study, the effect of SAL on the proliferation and osteogenic/odontogenic differentiation of human dental pulp stem cells (hDPSCs) was investigated. This was achieved by performing CCK-8 ARS staining assay, reverse transcription-quantitative PCR to detect mRNA of ALP, OSX, RUNX2, OCN, DSPP and BSP, western blotting to detect the protein of MAPK, Smad1/5/8, OSX, RUNX2, BSP and GAPDH and immunofluorescence assays to detect DSPP. The results indicated that SAL promoted the cell viability and the osteogenic/odontogenic differentiation of hDPSCs whilst increasing the expression of genes associated with osteogenic/odontogenic differentiation by ARS staining assay. In addition, SAL promoted osteogenic and odontogenic differentiation by activating the phosphorylation of Smad1/5/8. Collectively, these findings suggest that SAL promoted the osteogenic and odontogenic differentiation of hDPSCs activating the BMP signaling pathway.

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References

Chen Y, Zhao Q, Yang X, Yu X, Yu D, Zhao W . Effects of cobalt chloride on the stem cell marker expression and osteogenic differentiation of stem cells from human exfoliated deciduous teeth. Cell Stress Chaperones. 2019; 24(3):527-538. PMC: 6527733. DOI: 10.1007/s12192-019-00981-5. View

Wu A, Bao Y, Yu H, Zhou Y, Lu Q . Berberine Accelerates Odontoblast Differentiation by Wnt/β-Catenin Activation. Cell Reprogram. 2019; 21(2):108-114. DOI: 10.1089/cell.2018.0060. View

Ying Y, Luo J . Salidroside promotes human periodontal ligament cell proliferation and osteocalcin secretion via ERK1/2 and PI3K/Akt signaling pathways. Exp Ther Med. 2018; 15(6):5041-5045. PMC: 5952094. DOI: 10.3892/etm.2018.6006. View

Moonesi Rad R, Atila D, Akgun E, Evis Z, Keskin D, Tezcaner A . Evaluation of human dental pulp stem cells behavior on a novel nanobiocomposite scaffold prepared for regenerative endodontics. Mater Sci Eng C Mater Biol Appl. 2019; 100:928-948. DOI: 10.1016/j.msec.2019.03.022. View

Gomathi K, Akshaya N, Srinaath N, Moorthi A, Selvamurugan N . Regulation of Runx2 by post-translational modifications in osteoblast differentiation. Life Sci. 2020; 245:117389. DOI: 10.1016/j.lfs.2020.117389. View

Ahmed G, Abouauf E, AbuBakr N, Dorfer C, Fawzy El-Sayed K . Tissue Engineering Approaches for Enamel, Dentin, and Pulp Regeneration: An Update. Stem Cells Int. 2020; 2020:5734539. PMC: 7060883. DOI: 10.1155/2020/5734539. View

Chan W, Tan Z, To M, Chan D . Regulation and Role of Transcription Factors in Osteogenesis. Int J Mol Sci. 2021; 22(11). PMC: 8196788. DOI: 10.3390/ijms22115445. View

Miyazaki T, Kanatani N, Rokutanda S, Yoshida C, Toyosawa S, Nakamura R . Inhibition of the terminal differentiation of odontoblasts and their transdifferentiation into osteoblasts in Runx2 transgenic mice. Arch Histol Cytol. 2008; 71(2):131-46. DOI: 10.1679/aohc.71.131. View

Camilleri S, McDonald F . Runx2 and dental development. Eur J Oral Sci. 2006; 114(5):361-73. DOI: 10.1111/j.1600-0722.2006.00399.x. View

10.

Jung C, Kim S, Sun T, Cho Y, Song M . Pulp-dentin regeneration: current approaches and challenges. J Tissue Eng. 2019; 10:2041731418819263. PMC: 6351713. DOI: 10.1177/2041731418819263. View

11.

Liu K, Wang X, Li Y, Li B, Qi J, Zhang J . Tongxinluo Reverses the Hypoxia-suppressed Claudin-9 in Cardiac Microvascular Endothelial Cells. Chin Med J (Engl). 2016; 129(4):442-7. PMC: 4800845. DOI: 10.4103/0366-6999.176076. View

12.

DSouza R, Cavender A, Sunavala G, Alvarez J, Ohshima T, Kulkarni A . Gene expression patterns of murine dentin matrix protein 1 (Dmp1) and dentin sialophosphoprotein (DSPP) suggest distinct developmental functions in vivo. J Bone Miner Res. 1998; 12(12):2040-9. DOI: 10.1359/jbmr.1997.12.12.2040. View

13.

Shi R, Yang H, Lin X, Cao Y, Zhang C, Fan Z . Analysis of the characteristics and expression profiles of coding and noncoding RNAs of human dental pulp stem cells in hypoxic conditions. Stem Cell Res Ther. 2019; 10(1):89. PMC: 6417198. DOI: 10.1186/s13287-019-1192-2. View

14.

W Tsutsui T . Dental Pulp Stem Cells: Advances to Applications. Stem Cells Cloning. 2020; 13:33-42. PMC: 7025818. DOI: 10.2147/SCCAA.S166759. View

15.

Hao J, Yang H, Cao Y, Zhang C, Fan Z . IGFBP5 enhances the dentinogenesis potential of dental pulp stem cells via JNK and ErK signalling pathways. J Oral Rehabil. 2020; 47(12):1557-1565. DOI: 10.1111/joor.13047. View

16.

Yu A, Xu M, Yao P, Kwan K, Liu Y, Duan R . Corylin, a flavonoid derived from Psoralea Fructus, induces osteoblastic differentiation via estrogen and Wnt/β-catenin signaling pathways. FASEB J. 2020; 34(3):4311-4328. DOI: 10.1096/fj.201902319RRR. View

17.

Jin Q, Yuan K, Lin W, Niu C, Ma R, Huang Z . Comparative characterization of mesenchymal stem cells from human dental pulp and adipose tissue for bone regeneration potential. Artif Cells Nanomed Biotechnol. 2019; 47(1):1577-1584. DOI: 10.1080/21691401.2019.1594861. View

18.

Chen H, Fu H, Wu X, Duan Y, Zhang S, Hu H . Regeneration of pulpo-dentinal-like complex by a group of unique multipotent CD24a stem cells. Sci Adv. 2020; 6(15):eaay1514. PMC: 7141825. DOI: 10.1126/sciadv.aay1514. View

19.

Ran R, Yang H, Cao Y, Yan W, Jin L, Zheng Y . Depletion of EREG enhances the osteo/dentinogenic differentiation ability of dental pulp stem cells via the p38 MAPK and Erk pathways in an inflammatory microenvironment. BMC Oral Health. 2021; 21(1):314. PMC: 8215766. DOI: 10.1186/s12903-021-01675-0. View

20.

Komori T . Functions of Osteocalcin in Bone, Pancreas, Testis, and Muscle. Int J Mol Sci. 2020; 21(20). PMC: 7589887. DOI: 10.3390/ijms21207513. View