The Frequency and Consequences of Multipolar Mitoses in Undifferentiated Embryonic Stem Cells

Overview

Journal J Appl Biomed

Specialties Biomedical Engineering
General Medicine

Date 2021 Dec 15

PMID 34907719

Citations 1

Authors

Veronika Pospisilova

Milan Esner

Iveta Cervenkova

Radek Fedr

Jean-Yvez Tinevez

Ales Hampl

Martin Anger

Affiliations

Soon will be listed here.

Abstract

Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their clinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability.

Citing Articles

Geometric Control of Cell Behavior by Biomolecule Nanodistribution.

Pospisil J, Hrabovsky M, Bohaciakova D, Hovadkova Z, Jurasek M, Mlcouskova J ACS Biomater Sci Eng. 2022; 8(11):4789-4806.

PMID: 36202388 PMC: 9667466. DOI: 10.1021/acsbiomaterials.2c00650.

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