Identification of Two Anti-parallel Beta-sheet Conformations in the Solution Structure of Murine Epidermal Growth Factor by Proton Magnetic Resonance

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Nov 1

PMID 3490668

Citations 14

Authors

G T Montelione

K Wuthrich

E C Nice

A W Burgess

H A Scheraga

Affiliations

Soon will be listed here.

Abstract

Epidermal growth factor (EGF) is a small mitogenic protein. Proteins with sequence homology with EGF or with its membrane-bound protein receptor have been proposed to play a role in oncogenesis. This report describes solution NMR data that provide evidence that the solution conformation of murine EGF includes an anti-parallel beta-sheet structure involving residues S2-P4, V19-I23, and S28-N32; a small anti-parallel beta-sheet involving residues Y37-S38 and T44-R45; and a multiple-bend (or short irregular helix) structure for residues C6-C14 that is disulfide bonded to the V19-I23/S28-N32 beta-sheet. Implications of these results for structure and function studies of EGF and for molecular design of EGF and homologous alpha-type transforming growth factors are discussed.

Citing Articles

The significance of disulfide bonding in biological activity of HB-EGF, a mutagenesis approach.

Hoskins J, Zhou Z, Harding P Biochem Biophys Res Commun. 2008; 375(4):506-11.

PMID: 18725202 PMC: 2632935. DOI: 10.1016/j.bbrc.2008.08.062.

Structural dynamic of a self-assembling peptide d-EAK16 made of only D-amino acids.

Luo Z, Zhao X, Zhang S PLoS One. 2008; 3(5):e2364.

PMID: 18509542 PMC: 2387071. DOI: 10.1371/journal.pone.0002364.

Role of the 6-20 disulfide bridge in the structure and activity of epidermal growth factor.

Barnham K, Torres A, Alewood D, Alewood P, Domagala T, Nice E Protein Sci. 1999; 7(8):1738-49.

PMID: 10082370 PMC: 2144085. DOI: 10.1002/pro.5560070808.

Simulated annealing with restrained molecular dynamics using CONGEN: energy refinement of the NMR solution structures of epidermal and type-alpha transforming growth factors.

Tejero R, Bruccoleri R, Montelione G Protein Sci. 1996; 5(4):578-92.

PMID: 8845748 PMC: 2143379. DOI: 10.1002/pro.5560050403.

Structure-function studies of mEGF: probing the type I beta-turn between residues 25 and 26.

Lester C, Wang B, Wu R, Scheraga H J Protein Chem. 1995; 14(8):753-62.

PMID: 8747437 DOI: 10.1007/BF01886915.

References

Smith J, Sporn M, Roberts A, Derynck R, Winkler M, Gregory H . Human transforming growth factor-alpha causes precocious eyelid opening in newborn mice. Nature. 1985; 315(6019):515-6. DOI: 10.1038/315515a0. View

Savage Jr C, Hash J, Cohen S . Epidermal growth factor. Location of disulfide bonds. J Biol Chem. 1973; 248(22):7669-72. View

Cohen S . Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal. J Biol Chem. 1962; 237:1555-62. View

Mayo K . Epidermal growth factor from the mouse. Physical evidence for a tiered beta-sheet domain: two-dimensional NMR correlated spectroscopy and nuclear Overhauser experiments on backbone amide protons. Biochemistry. 1985; 24(14):3783-94. DOI: 10.1021/bi00335a055. View

Wagner G, Wuthrich K . Sequential resonance assignments in protein 1H nuclear magnetic resonance spectra. Basic pancreatic trypsin inhibitor. J Mol Biol. 1982; 155(3):347-66. DOI: 10.1016/0022-2836(82)90009-2. View

De Larco J, Todaro G . Growth factors from murine sarcoma virus-transformed cells. Proc Natl Acad Sci U S A. 1978; 75(8):4001-5. PMC: 392918. DOI: 10.1073/pnas.75.8.4001. View

Williamson M, Marion D, Wuthrich K . Secondary structure in the solution conformation of the proteinase inhibitor IIA from bull seminal plasma by nuclear magnetic resonance. J Mol Biol. 1984; 173(3):341-59. DOI: 10.1016/0022-2836(84)90125-6. View

Todaro G, Fryling C, De Larco J . Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors. Proc Natl Acad Sci U S A. 1980; 77(9):5258-62. PMC: 350037. DOI: 10.1073/pnas.77.9.5258. View

Brousseau R, Scarpulla R, Sung W, Hsiung H, Narang S, Wu R . Synthesis of a human insulin gene. V. Enzymatic assembly, cloning and characterization of the human proinsulin DNA. Gene. 1982; 17(3):279-89. DOI: 10.1016/0378-1119(82)90144-5. View

10.

Burgess A, Knesel J, Sparrow L, Nicola N, Nice E . Two forms of murine epidermal growth factor: rapid separation by using reverse-phase HPLC. Proc Natl Acad Sci U S A. 1982; 79(19):5753-7. PMC: 346988. DOI: 10.1073/pnas.79.19.5753. View

11.

Banyai L, Varadi A, Patthy L . Common evolutionary origin of the fibrin-binding structures of fibronectin and tissue-type plasminogen activator. FEBS Lett. 1983; 163(1):37-41. DOI: 10.1016/0014-5793(83)81157-0. View

12.

Savage Jr C, Inagami T, Cohen S . The primary structure of epidermal growth factor. J Biol Chem. 1972; 247(23):7612-21. View

13.

Komoriya A, Hortsch M, Meyers C, Smith M, Kanety H, Schlessinger J . Biologically active synthetic fragments of epidermal growth factor: localization of a major receptor-binding region. Proc Natl Acad Sci U S A. 1984; 81(5):1351-5. PMC: 344831. DOI: 10.1073/pnas.81.5.1351. View

14.

Gregory H . Isolation and structure of urogastrone and its relationship to epidermal growth factor. Nature. 1975; 257(5524):325-7. DOI: 10.1038/257325a0. View

15.

Zuiderweg E, Kaptein R, Wuthrich K . Secondary structure of the lac repressor DNA-binding domain by two-dimensional 1H nuclear magnetic resonance in solution. Proc Natl Acad Sci U S A. 1983; 80(19):5837-41. PMC: 390170. DOI: 10.1073/pnas.80.19.5837. View

16.

Wagner G, Neuhaus D, Worgotter E, Vasak M, KAGI J, Wuthrich K . Nuclear magnetic resonance identification of "half-turn" and 3(10)-helix secondary structure in rabbit liver metallothionein-2. J Mol Biol. 1986; 187(1):131-5. DOI: 10.1016/0022-2836(86)90413-4. View

17.

Marion D, Wuthrich K . Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins. Biochem Biophys Res Commun. 1983; 113(3):967-74. DOI: 10.1016/0006-291x(83)91093-8. View

18.

Sporn M, Roberts A . Autocrine growth factors and cancer. Nature. 1985; 313(6005):745-7. DOI: 10.1038/313745a0. View

19.

Kline A, Wuthrich K . Secondary structure of the alpha-amylase polypeptide inhibitor tendamistat from Streptomyces tendae determined in solution by 1H nuclear magnetic resonance. J Mol Biol. 1985; 183(3):503-7. DOI: 10.1016/0022-2836(85)90018-x. View

20.

Wagner G, Zuiderweg E . Two-dimensional double quantum 1H NMR spectroscopy of proteins. Biochem Biophys Res Commun. 1983; 113(3):854-60. DOI: 10.1016/0006-291x(83)91077-x. View