M-MuLV Reverse Transcriptase: Selected Properties and Improved Mutants

Overview

Journal Comput Struct Biotechnol J

Specialty Biotechnology

Date 2021 Dec 13

PMID 34900141

Citations 13

Authors

Igor P Oscorbin

Maxim L Filipenko

Affiliations

Soon will be listed here.

Abstract

Reverse transcriptases (RTs) are enzymes synthesizing DNA using RNA as the template and serving as the standard tools in modern biotechnology and molecular diagnostics. To date, the most commonly used reverse transcriptase is the enzyme from Moloney murine leukemia virus, M-MuLV RT. Since its discovery, M-MuLV RT has become indispensable for modern RNA studies; the range of M-MuLV RT applications is vast, from scientific tasks to clinical testing of human pathogens. This review will give a brief description of the structure, thermal stability, processivity, and fidelity, focusing on improving M-MuLV RT for practical usage.

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