Biological Response Profiling Reveals the Functional Differences of Main Alkaloids in Rhizoma Coptidis

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Dec 10

PMID 34885971

Citations 4

Authors

Lan Xie

Shanshan Feng

Xiaoling Zhang

Wenlong Zhao

Juan Feng

Chengmei Ma

Ruijun Wang

Weifang Song

Jing Cheng

Affiliations

Soon will be listed here.

Abstract

Rhizoma Coptidis (RC) is a widely used traditional Chinese medicine. Although modern research has found that some alkaloids from RC are the pharmacologically active constituents, the differences in their biological effects are not completely clear. This study analyzed the differences in the typical alkaloids in RC at a systematic level and provided comprehensive information on the pharmaceutical mechanisms of the different alkaloids. The ethanol RC extract (RCE) was characterized using HPLC assay. HepG2, 3T3-L1, and RAW264.7 cells were used to detect the cytotoxicity of alkaloids. Transcriptome analyses were performed to elucidate the cellular pathways affected by RCE and alkaloids. HPLC analysis revealed that the typical alkaloids of RCE were berberine, coptisine, and palmatine. Coptisine and berberine displayed a stronger inhibitory effect on cell proliferation than palmatine. The overlapping ratios of differentially expressed genes between RCE and berberine, coptisine, and palmatine were 70.8%, 52.6%, and 42.1%, respectively. Pathway clustering analysis indicated that berberine and coptisine possessed a certain similarity to RCE, and both compounds affected the cell cycle pathway; moreover, some pathways were uniquely enriched by berberine or coptisine. Berberine and coptisine had different regulatory effects on genes involved in lipid metabolism. These results provide comprehensive information on the pharmaceutical mechanisms of the different RC alkaloids and insights into their better combinatory use for the treatment of diseases.

Citing Articles

Liquid chromatography-tandem mass spectrometry analysis of a ratio-optimized drug pair of Aiton and Franch and study on the mechanism of anti-colorectal cancer effect of two alkaloids thereof.

Chen Z, Dong Y, Yan Q, Li Q, Yu C, Lai Y Front Oncol. 2023; 13:1198467.

PMID: 37404762 PMC: 10316516. DOI: 10.3389/fonc.2023.1198467.

An Ethanol Extract of Induces Apoptotic Cell Death in Induced Pluripotent Stem Cells and Suppresses Teratoma Formation.

Kim A, Baek S, Shin S, Lee S, Chung S Nutrients. 2023; 15(10).

PMID: 37242247 PMC: 10221726. DOI: 10.3390/nu15102364.

Oral administration of Huanglian-Houpo herbal nanoemulsion loading multiple phytochemicals for ulcerative colitis therapy in mice.

Wang X, Fu L, Cheng W, Chen J, Zhang H, Zhu H Drug Deliv. 2023; 30(1):2204207.

PMID: 37139554 PMC: 10332195. DOI: 10.1080/10717544.2023.2204207.

Isoquinoline Alkaloids from Franch: Focus on Coptisine as a Potential Therapeutic Candidate against Gastric Cancer Cells.

Nakonieczna S, Grabarska A, Gawel K, Wroblewska-Luczka P, Czerwonka A, Stepulak A Int J Mol Sci. 2022; 23(18).

PMID: 36142236 PMC: 9499618. DOI: 10.3390/ijms231810330.

References

Yi J, Ye X, Wang D, He K, Yang Y, Liu X . Safety evaluation of main alkaloids from Rhizoma Coptidis. J Ethnopharmacol. 2012; 145(1):303-10. DOI: 10.1016/j.jep.2012.10.062. View

Ning N, He K, Wang Y, Zou Z, Wu H, Li X . Hypolipidemic Effect and Mechanism of Palmatine from Coptis chinensis in Hamsters Fed High-Fat diet. Phytother Res. 2015; 29(5):668-73. DOI: 10.1002/ptr.5295. View

Yan B, Wang D, Dong S, Cheng Z, Na L, Sang M . Palmatine inhibits TRIF-dependent NF-κB pathway against inflammation induced by LPS in goat endometrial epithelial cells. Int Immunopharmacol. 2017; 45:194-200. DOI: 10.1016/j.intimp.2017.02.004. View

Huang P, Qian X, Li J, Cui X, Chen L, Cai B . Simultaneous determination of 11 alkaloids in crude and wine-processed Rhizoma Coptidis by HPLC-PAD. J Chromatogr Sci. 2014; 53(1):73-8. DOI: 10.1093/chromsci/bmu019. View

Wang N, Tan H, Li L, Yuen M, Feng Y . Berberine and Coptidis Rhizoma as potential anticancer agents: Recent updates and future perspectives. J Ethnopharmacol. 2015; 176:35-48. DOI: 10.1016/j.jep.2015.10.028. View

Kou S, Han B, Wang Y, Huang T, He K, Han Y . Synergetic cholesterol-lowering effects of main alkaloids from Rhizoma Coptidis in HepG2 cells and hypercholesterolemia hamsters. Life Sci. 2016; 151:50-60. DOI: 10.1016/j.lfs.2016.02.046. View

Chai F, Ma W, Zhang J, Xu H, Li Y, Zhou Q . Coptisine from Rhizoma coptidis exerts an anti-cancer effect on hepatocellular carcinoma by up-regulating miR-122. Biomed Pharmacother. 2018; 103:1002-1011. DOI: 10.1016/j.biopha.2018.04.052. View

Piper M, Gronostajski R, Messina G . Nuclear Factor One X in Development and Disease. Trends Cell Biol. 2018; 29(1):20-30. DOI: 10.1016/j.tcb.2018.09.003. View

Rong J, Tilton R, Shen J, Ng K, Liu C, Tam P . Genome-wide biological response fingerprinting (BioReF) of the Chinese botanical formulation ISF-1 enables the selection of multiple marker genes as a potential metric for quality control. J Ethnopharmacol. 2007; 113(1):35-44. DOI: 10.1016/j.jep.2007.01.021. View

10.

Santamaria D, Barriere C, Cerqueira A, Hunt S, Tardy C, Newton K . Cdk1 is sufficient to drive the mammalian cell cycle. Nature. 2007; 448(7155):811-5. DOI: 10.1038/nature06046. View

11.

Tarabasz D, Kukula-Koch W . Palmatine: A review of pharmacological properties and pharmacokinetics. Phytother Res. 2019; 34(1):33-50. DOI: 10.1002/ptr.6504. View

12.

Wu J, Zhang H, Hu B, Yang L, Wang P, Wang F . Coptisine from Coptis chinensis inhibits production of inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 murine macrophage cells. Eur J Pharmacol. 2016; 780:106-14. DOI: 10.1016/j.ejphar.2016.03.037. View

13.

Kong W, Wei J, Abidi P, Lin M, Inaba S, Li C . Berberine is a novel cholesterol-lowering drug working through a unique mechanism distinct from statins. Nat Med. 2004; 10(12):1344-51. DOI: 10.1038/nm1135. View

14.

Wang H, Mu W, Shang H, Lin J, Lei X . The antihyperglycemic effects of Rhizoma Coptidis and mechanism of actions: a review of systematic reviews and pharmacological research. Biomed Res Int. 2014; 2014:798093. PMC: 4003828. DOI: 10.1155/2014/798093. View

15.

Shi L, Jia W, Zhang L, Xu C, Chen X, Yin L . Glucose consumption assay discovers coptisine with beneficial effect on diabetic mice. Eur J Pharmacol. 2019; 859:172523. DOI: 10.1016/j.ejphar.2019.172523. View

16.

Meng F, Wu Z, Yin Z, Lin L, Wang R, Zhang Q . Coptidis rhizoma and its main bioactive components: recent advances in chemical investigation, quality evaluation and pharmacological activity. Chin Med. 2018; 13:13. PMC: 5842587. DOI: 10.1186/s13020-018-0171-3. View

17.

Takatori H, Yamashita T, Honda M, Nishino R, Arai K, Yamashita T . dUTP pyrophosphatase expression correlates with a poor prognosis in hepatocellular carcinoma. Liver Int. 2009; 30(3):438-46. DOI: 10.1111/j.1478-3231.2009.02177.x. View

18.

Galmarini C, Cros E, Graham K, Thomas X, Mackey J, Dumontet C . 5'-(3')-nucleotidase mRNA levels in blast cells are a prognostic factor in acute myeloid leukemia patients treated with cytarabine. Haematologica. 2004; 89(5):617-9. View

19.

He K, Ye X, Wu H, Wang Y, Zou Z, Ning N . The safety and anti-hypercholesterolemic effect of coptisine in Syrian golden hamsters. Lipids. 2014; 50(2):185-94. DOI: 10.1007/s11745-014-3983-7. View

20.

Zou K, Li Z, Zhang Y, Zhang H, Li B, Zhu W . Advances in the study of berberine and its derivatives: a focus on anti-inflammatory and anti-tumor effects in the digestive system. Acta Pharmacol Sin. 2016; 38(2):157-167. PMC: 5309756. DOI: 10.1038/aps.2016.125. View