Measurement of the Drug Sensitivity of Single Prostate Cancer Cells

Overview

Journal Cancers (Basel)

Publisher MDPI

Specialty Oncology

Date 2021 Dec 10

PMID 34885192

Citations 2

Authors

Fikri Abali

Narges Baghi

Lisanne Mout

Joska J Broekmaat

Arjan G J Tibbe

Leon W M M Terstappen

Affiliations

Soon will be listed here.

Abstract

The treatment of cancer faces a serious challenge as cancer cells within patients are heterogeneous and frequently resistant to therapeutic drugs. Here, we introduce a technology enabling the assessment of single cancer cells exposed to different drugs. PCa cells were individually sorted in self-seeding microwells, cultured for 24 h, and then exposed to several drugs to induce (R1881) or inhibit (Enzalutamide/Abiraterone) the secretion of a protein (PSA). Cell viability and PSA secretion of each individual prostate cell were monitored over a 3-day period. The PSA protein secreted by each cell was captured on a PVDF membrane through a pore in the bottom of each well. The basal PSA secretion was found to be 6.1 ± 4.5 and 3.7 ± 1.9 pg/cell/day for LNCaP and VCaP, respectively. After exposure to R1881, the PSA secretion increased by ~90% on average and was not altered for ~10% of the cells. PSA production decreased in the majority of cells after exposure to enzalutamide and abiraterone.

Citing Articles

Secretome Analysis of Prostate Cancer Cell Lines Reveals Cell Cycle-Dependent PSA Secretion and Potential Biomarkers.

Dathathri E, Peters Y, Andreoli D, Bruins M, Kraan J, Terstappen L Cancers (Basel). 2025; 17(5).

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Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.

Wang C, Qiu J, Liu M, Wang Y, Yu Y, Liu H Adv Sci (Weinh). 2024; 11(28):e2401263.

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Liquid Biopsy Based Circulating Biomarkers in Metastatic Prostate Cancer.

Dathathri E, Isebia K, Abali F, Lolkema M, Martens J, Terstappen L Front Oncol. 2022; 12:863472.

PMID: 35669415 PMC: 9165750. DOI: 10.3389/fonc.2022.863472.

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