Transcriptome Analysis Reveals Sexual Disparities Between Olfactory and Immune Gene Expression in the Olfactory Epithelium of

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Dec 10

PMID 34884822

Citations 1

Authors

Maolin Lv

Xiuli Chen

Xin Huang

Ning Liu

Weimin Wang

Han Liu

Affiliations

Soon will be listed here.

Abstract

The olfactory organ is an important chemoreceptor in vertebrates. However, the sexual disparities in gene expression patterns in the olfactory organ in fish remain unstudied. Here, we conducted a transcriptome analysis of the olfactory epithelium (OE) of male and female blunt snout bream (Megalobrama amblycephala) to identify the differences. The histological analysis showed that there were 22 leaf-like olfactory lamellaes on one side of the OE of the adult blunt snout bream. The sensory area of OE is enriched with ciliated receptor cells and microvilli receptor cells. The transcriptome analysis showed that only 10 out of 336 olfactory receptor genes (224 ORs, 5 V1Rs, 55 V2Rs, and 52 TAARs) exhibited significant expression differences between males and females, and most of the differentially expressed genes were related to the immune system. We also validated these results using qPCR: 10 OR genes and 6 immunity-related genes significantly differed between males and females. The FISH analysis results indicated that the ORs were mainly expressed at the edge of the olfactory lamellae. Collectively, our study reveals that gender is not an important factor influencing the expression of olfactory receptors, but the expression of immune genes varies greatly between the genders in blunt snout bream.

Citing Articles

Full-length transcriptome assembly of black amur bream (Megalobrama terminalis) as a reference resource.

Liu K, Xie N Mol Biol Rep. 2024; 51(1):1101.

PMID: 39470845 DOI: 10.1007/s11033-024-10056-z.

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