Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2021 Dec 10

PMID 34883850

Citations 4

Authors

Anastasiia V Shabalina

Darya O Sharko

Yury E Glazyrin

Elena A Bolshevich

Oksana V Dubinina

Anastasiia M Kim

Dmitry V Veprintsev

Ivan N Lapin

Galina S Zamay

Alexey V Krat

Sergey S Zamay

Valery A Svetlichnyi

Anna S Kichkailo

Maxim V Berezovski

Affiliations

Soon will be listed here.

Abstract

We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fill the aptamer layer's defects. Electrochemical aptasensor signal processing was enhanced using deep learning and computer simulation of the experimental data array. It was found that the combinations (coupled and tripled) of cyclic voltammogram features allowed for distinguishing between the samples from lung cancer patients and healthy candidates with a mean accuracy of 0.73. The capacitive component from the non-Faradic electrochemical impedance spectroscopy data indicated the tumor marker's presence in a sample. These findings allowed for the creation of highly informative aptasensors for early lung cancer diagnostics.

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References

Negahdary M . Electrochemical aptasensors based on the gold nanostructures. Talanta. 2020; 216:120999. DOI: 10.1016/j.talanta.2020.120999. View

Akhtartavan S, Karimi M, Sattarahmady N, Heli H . An electrochemical signal-on apta-cyto-sensor for quantitation of circulating human MDA-MB-231 breast cancer cells by transduction of electro-deposited non-spherical nanoparticles of gold. J Pharm Biomed Anal. 2019; 178:112948. DOI: 10.1016/j.jpba.2019.112948. View

Khosropour H, Rezaei B, Rezaei P, Ensafi A . Ultrasensitive voltammetric and impedimetric aptasensor for diazinon pesticide detection by VS quantum dots-graphene nanoplatelets/carboxylated multiwalled carbon nanotubes as a new group nanocomposite for signal enrichment. Anal Chim Acta. 2020; 1111:92-102. DOI: 10.1016/j.aca.2020.03.047. View

Taghdisi S, Danesh N, Nameghi M, Ramezani M, Alibolandi M, Hassanzadeh-Khayat M . A novel electrochemical aptasensor based on nontarget-induced high accumulation of methylene blue on the surface of electrode for sensing of α-synuclein oligomer. Biosens Bioelectron. 2018; 123:14-18. DOI: 10.1016/j.bios.2018.09.081. View

Bai Z, Chen Y, Li F, Zhou Y, Yin H, Ai S . Electrochemical aptasensor for sulfadimethoxine detection based on the triggered cleavage activity of nuclease P1 by aptamer-target complex. Talanta. 2019; 204:409-414. DOI: 10.1016/j.talanta.2019.06.035. View

McKeown S, Lundy F, Nelson J, Lockhart D, Irwin C, Cowan C . The cytotoxic effects of human neutrophil peptide-1 (HNP1) and lactoferrin on oral squamous cell carcinoma (OSCC) in vitro. Oral Oncol. 2006; 42(7):685-90. DOI: 10.1016/j.oraloncology.2005.11.005. View

Abnous K, Danesh N, Nameghi M, Ramezani M, Alibolandi M, Lavaee P . An ultrasensitive electrochemical sensing method for detection of microcystin-LR based on infinity-shaped DNA structure using double aptamer and terminal deoxynucleotidyl transferase. Biosens Bioelectron. 2019; 144:111674. DOI: 10.1016/j.bios.2019.111674. View

Bagheri Hashkavayi A, Raoof J . Ultrasensitive and reusable electrochemical aptasensor for detection of tryptophan using of [Fe(bpy)](p-CHCHSO) as an electroactive indicator. J Pharm Biomed Anal. 2018; 163:180-187. DOI: 10.1016/j.jpba.2018.10.006. View

Siller I, Preuss J, Urmann K, Hoffmann M, Scheper T, Bahnemann J . 3D-Printed Flow Cells for Aptamer-Based Impedimetric Detection of Crooks Strain. Sensors (Basel). 2020; 20(16). PMC: 7472219. DOI: 10.3390/s20164421. View

10.

Zamay G, Kolovskaya O, Zamay T, Glazyrin Y, Krat A, Zubkova O . Aptamers Selected to Postoperative Lung Adenocarcinoma Detect Circulating Tumor Cells in Human Blood. Mol Ther. 2015; 23(9):1486-96. PMC: 4817883. DOI: 10.1038/mt.2015.108. View

11.

Holterman D, Diaz J, Blackmore P, Davis J, Schellhammer P, Corica A . Overexpression of alpha-defensin is associated with bladder cancer invasiveness. Urol Oncol. 2006; 24(2):97-108. DOI: 10.1016/j.urolonc.2005.07.010. View

12.

Nan M, Bi Y, Xue H, Long H, Xue S, Pu L . Modification performance and electrochemical characteristics of different groups of modified aptamers applied for label-free electrochemical impedimetric sensors. Food Chem. 2020; 337:127761. DOI: 10.1016/j.foodchem.2020.127761. View

13.

Espinosa J . Histone H2B ubiquitination: the cancer connection. Genes Dev. 2008; 22(20):2743-9. PMC: 2751279. DOI: 10.1101/gad.1732108. View

14.

Ghanbari K, Roushani M, Soheyli E, Sahraei R . An electrochemical tyrosinamide aptasensor using a glassy carbon electrode modified by N-acetyl-l-cysteine-capped Ag-In-S QDs. Mater Sci Eng C Mater Biol Appl. 2019; 102:653-660. DOI: 10.1016/j.msec.2019.04.093. View

15.

Hongxia C, Zaijun L, Ruiyi L, Guangli W, Zhiguo G . Molecular machine and gold/graphene quantum dot hybrid based dual amplification strategy for voltammetric detection of VEGF165. Mikrochim Acta. 2019; 186(4):242. DOI: 10.1007/s00604-019-3336-6. View

16.

Gustavsson T, Markovitsi D . Fundamentals of the Intrinsic DNA Fluorescence. Acc Chem Res. 2021; 54(5):1226-1235. DOI: 10.1021/acs.accounts.0c00603. View

17.

Cole A, Clifton-Bligh R, Marsh D . Histone H2B monoubiquitination: roles to play in human malignancy. Endocr Relat Cancer. 2014; 22(1):T19-33. DOI: 10.1530/ERC-14-0185. View

18.

An Y, Jin T, Zhu Y, Zhang F, He P . An ultrasensitive electrochemical aptasensor for the determination of tumor exosomes based on click chemistry. Biosens Bioelectron. 2019; 142:111503. DOI: 10.1016/j.bios.2019.111503. View

19.

Guo W, Zhang C, Ma T, Liu X, Chen Z, Li S . Advances in aptamer screening and aptasensors' detection of heavy metal ions. J Nanobiotechnology. 2021; 19(1):166. PMC: 8171055. DOI: 10.1186/s12951-021-00914-4. View

20.

An K, Lu X, Wang C, Qian J, Chen Q, Hao N . Porous Gold Nanocages: High Atom Utilization for Thiolated Aptamer Immobilization to Well Balance the Simplicity, Sensitivity, and Cost of Disposable Aptasensors. Anal Chem. 2019; 91(13):8660-8666. DOI: 10.1021/acs.analchem.9b02145. View