Development of Electrochemical Aptasensor for Lung Cancer Diagnostics in Human Blood
Overview
Authors
Affiliations
We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fill the aptamer layer's defects. Electrochemical aptasensor signal processing was enhanced using deep learning and computer simulation of the experimental data array. It was found that the combinations (coupled and tripled) of cyclic voltammogram features allowed for distinguishing between the samples from lung cancer patients and healthy candidates with a mean accuracy of 0.73. The capacitive component from the non-Faradic electrochemical impedance spectroscopy data indicated the tumor marker's presence in a sample. These findings allowed for the creation of highly informative aptasensors for early lung cancer diagnostics.
Aptamers Enhance Oncolytic Viruses' Antitumor Efficacy.
Dymova M, Kichkailo A, Kuligina E, Richter V Pharmaceutics. 2023; 15(1).
PMID: 36678780 PMC: 9864469. DOI: 10.3390/pharmaceutics15010151.
Current Update on Biomarkers for Detection of Cancer: Comprehensive Analysis.
Kaushal A, Kaur N, Sharma S, Sharma A, Kala D, Prakash H Vaccines (Basel). 2022; 10(12).
PMID: 36560548 PMC: 9787556. DOI: 10.3390/vaccines10122138.
Recent Advances in Electrochemical Aptasensors for Detection of Biomarkers.
Majdinasab M, Marty J Pharmaceuticals (Basel). 2022; 15(8).
PMID: 36015143 PMC: 9412480. DOI: 10.3390/ph15080995.
Cancer Diagnostics and Early Detection Using Electrochemical Aptasensors.
Omage J, Easterday E, Rumph J, Brula I, Hill B, Kristensen J Micromachines (Basel). 2022; 13(4).
PMID: 35457828 PMC: 9026785. DOI: 10.3390/mi13040522.