Molecular Mimicry in Reiter's Syndrome: Cytotoxicity and ELISA Studies of HLA-microbial Relationships

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1986 Jul 1

PMID 3488263

Citations 5

Authors

R D Inman

B Chiu

M E Johnston

J Falk

Affiliations

Soon will be listed here.

Abstract

The pathogenic links between HLA antigens, certain bacterial infections and arthritis have not yet been characterized. The hypothesis of cross-reactivity between HLA B27, the marker of disease susceptibility for these disorders, and the provocative microorganism has been suggested by studies of Klebsiella and ankylosing spondylitis. The present study examines the possibility of molecular mimicry between HLA B27 and two organisms implicated more directly in reactive arthritis, Yersinia enterocolitica and Chlamydia trachomatis. Antibodies against these organisms were obtained both from patients and from antisera raised in rabbits. Neither source of antibacterial antibody was specifically cytotoxic for HLA B27-positive lymphocytes, even when the target cells were derived from patients with recent infections due to these organisms. In addition, monoclonal antibodies against HLA B27 (M1 and M2) showed no reactivity with antigens from these organisms in an ELISA system. These data do not support the notion of molecular mimicry as being the basis of immunogenetic susceptibility to reactive arthritis and Reiter's syndrome following infections with Y. enterocolitica and C. trachomatis.

Citing Articles

Yersiniosis in New Zealand.

Rivas L, Strydom H, Paine S, Wang J, Wright J Pathogens. 2021; 10(2).

PMID: 33578727 PMC: 7916520. DOI: 10.3390/pathogens10020191.

HLA-B27 associated spondyloarthropathy, an autoimmune disease based on crossreactivity between bacteria and HLA-B27?.

Ringrose J Ann Rheum Dis. 1999; 58(10):598-610.

PMID: 10491358 PMC: 1752789. DOI: 10.1136/ard.58.10.598.

Immunochemical analysis of immune response to Chlamydia trachomatis in Reiter's syndrome and nonspecific urethritis.

Inman R, Johnston M, Chiu B, Falk J, Petric M Clin Exp Immunol. 1987; 69(2):246-54.

PMID: 3652532 PMC: 1542407.

Autoantibodies to the HLA-B27 sequence cross-react with the hypothetical peptide from the arthritis-associated Shigella plasmid.

Tsuchiya N, Husby G, Williams Jr R, STIEGLITZ H, Lipsky P, Inman R J Clin Invest. 1990; 86(4):1193-203.

PMID: 2212008 PMC: 296849. DOI: 10.1172/JCI114825.

HLA-B27/microbial mimicry: an in vivo analysis.

Kapasi K, Chui B, Inman R Immunology. 1992; 77(3):456-61.

PMID: 1478690 PMC: 1421722.

References

Zabriskie J, FREIMER E . An immunological relationship between the group. A streptococcus and mammalian muscle. J Exp Med. 1966; 124(4):661-78. PMC: 2138256. DOI: 10.1084/jem.124.4.661. View

Kono D, Ogasawara M, Effros R, Park M, Waldord R, Yu D . Ye-1, a monoclonal antibody that cross-reacts with HLA-B27 lymphoblastoid cell lines and an arthritis causing bacteria. Clin Exp Immunol. 1985; 61(3):503-8. PMC: 1577260. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Geczy A, Alexander K, Bashir H, Edmonds J . A factor(s) in Klebsiella culture filtrates specifically modifies an HLA-B27 associated cell-surface component. Nature. 1980; 283(5749):782-4. DOI: 10.1038/283782a0. View

Granfors K, Viljanen M, TIILIKAINEN A, Toivanen A . Persistence of IgM, IgG, and IgA antibodies to Yersinia in yersinia arthritis. J Infect Dis. 1980; 141(4):424-9. DOI: 10.1093/infdis/141.4.424. View

Warren R, BREWERTON D . Faecal carriage of klebsiella by patients with ankylosing spondylitis and rheumatoid arthritis. Ann Rheum Dis. 1980; 39(1):37-44. PMC: 1000467. DOI: 10.1136/ard.39.1.37. View

Avakian H, Welsh J, Ebringer A, ENTWISTLE C . Ankylosing spondylitis, HLA-B27 and Klebsiella. II. Cross-reactivity studies with human tissue typing sera. Br J Exp Pathol. 1980; 61(1):92-6. PMC: 2041554. View

Lane D, Hoeffler W . SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000. Nature. 1980; 288(5787):167-70. DOI: 10.1038/288167a0. View

Keat A, Thomas B, Taylor-Robinson D, Pegrum G, Maini R, SCOTT J . Evidence of Chlamydia trachomatis infection in sexually acquired reactive arthritis. Ann Rheum Dis. 1980; 39(5):431-7. PMC: 1000580. DOI: 10.1136/ard.39.5.431. View

10.

Grumet F, Fendly B, Engleman E . Monoclonal anti-HLA-B27 antibody (B27M1): production and lack of detectable typing difference between patients with ankylosing spondylitis, Reiter's syndrome, and normal controls. Lancet. 1981; 2(8239):174-6. DOI: 10.1016/s0140-6736(81)90358-5. View

11.

Grumet F, Fendly B, Fish L, Foung S, Engleman E . Monoclonal antibody (B27M2) subdividing HLA-B27. Hum Immunol. 1982; 5(1):61-72. DOI: 10.1016/0198-8859(82)90031-3. View

12.

Beaulieu A, Rousseau F, Israel-Assayag E, Roy R . Klebsiella related antigens in ankylosing spondylitis. J Rheumatol. 1983; 10(1):102-5. View

13.

CAMERON F, Russell P, Sullivan J, Geczy A . Is a Klebsiella plasmid involved in the aetiology of Ankylosing Spondylitis in HLA-B27-positive individuals?. Mol Immunol. 1983; 20(5):563-6. DOI: 10.1016/0161-5890(83)90095-0. View

14.

LEVY N, Munoz A, McCormack W . Enzyme-linked immunosorbent assay for the detection of antibody to Chlamydia trachomatis and Chlamydia psittaci. J Lab Clin Med. 1983; 102(6):918-25. View

15.

Keat A . Reiter's syndrome and reactive arthritis in perspective. N Engl J Med. 1983; 309(26):1606-15. DOI: 10.1056/NEJM198312293092604. View

16.

Martin D, Pollock S, Kuo C, Wang S, Brunham R, Holmes K . Chlamydia trachomatis infections in men with Reiter's syndrome. Ann Intern Med. 1984; 100(2):207-13. DOI: 10.7326/0003-4819-100-2-207. View

17.

Brenner M, Kobayashi S, Wiesenhutter C, Huberman A, Bales P, Yu D . In vitro T lymphocyte proliferative response to Yersinia enterocolitica in Reiter's syndrome. Lack of response in other HLA-B27 positive individuals. Arthritis Rheum. 1984; 27(3):250-7. DOI: 10.1002/art.1780270302. View

18.

Shinebaum R, Neumann V, Hopkins R, Cooke E, WRIGHT V . Attempt to modify klebsiella carriage in ankylosing spondylitic patients by diet: correlation of klebsiella carriage with disease activity. Ann Rheum Dis. 1984; 43(2):196-9. PMC: 1001464. DOI: 10.1136/ard.43.2.196. View

19.

van Bohemen C, Grumet F, Zanen H . Identification of HLA-B27M1 and -M2 cross-reactive antigens in Klebsiella, Shigella and Yersinia. Immunology. 1984; 52(4):607-10. PMC: 1454655. View

20.

Prendergast J, McGuigan L, Geczy A, Kwong T, Edmonds J . Persistence of HLA-B27 cross-reactive bacteria in bowel flora of patients with ankylosing spondylitis. Infect Immun. 1984; 46(3):686-9. PMC: 261598. DOI: 10.1128/iai.46.3.686-689.1984. View