T-cell Receptor Variable Region Gene Usage in T-cell Populations

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Jun 1

PMID 3487085

Citations 21

Authors

R D Garman

J L Ko

C D Vulpe

D H Raulet

Affiliations

Soon will be listed here.

Abstract

We have examined T-cell receptor alpha- and beta-chain variable (V) region gene usage in T-cell populations predicted to have different major histocompatibility complex-restriction specificities. Using a sensitive ribonuclease protection assay to measure T-cell receptor mRNA levels, we found no striking differences in the usage of three V alpha genes and three V beta genes in T-cell populations from three congeneic H-2-disparate strains of mice and between the mutually exclusive Ly2+ L3T4- and Ly2- L3T4+ T-cell subpopulations. These results suggest that major histocompatibility complex restriction cannot be explained by the differential usage of nonoverlapping V alpha or V beta gene pools. In contrast, striking but unpredictable differences were seen in V gene usage in populations of T cells selected by activation with particular alloantigens.

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