Distinct Physiological Roles of Three Phospholipid:Diacylglycerol Acyltransferase Genes in Olive Fruit with Respect to Oil Accumulation and the Response to Abiotic Stress

Overview

Journal Front Plant Sci

Date 2021 Dec 6

PMID 34868139

Citations 5

Authors

M Luisa Hernandez

Samuele Moretti

M Dolores Sicardo

Ursula Garcia

Ana Perez

Luca Sebastiani

Jose M Martinez-Rivas

Affiliations

Soon will be listed here.

Abstract

Three different cDNA sequences, designated , , and , encoding three phospholipid:diacylglycerol acyltransferases (PDAT) have been isolated from olive ( cv. Picual). Sequence analysis showed the distinctive features typical of the PDAT family and together with phylogenetic analysis indicated that they encode PDAT. Gene expression analysis in different olive tissues showed that transcript levels of these three genes are spatially and temporally regulated and suggested that, in addition to acyl-CoA:diacylglycerol acyltransferase, may contribute to the biosynthesis of triacylglycerols in the seed, whereas could be involved in the triacylglycerols content in the mesocarp and, therefore, in the olive oil. The relative contribution of PDAT and acyl-CoA:diacylglycerol acyltransferase enzymes to the triacylglycerols content in olive appears to be tissue-dependent. Furthermore, water regime, temperature, light, and wounding regulate genes at transcriptional level in the olive fruit mesocarp, indicating that PDAT could be involved in the response to abiotic stresses. Altogether, this study represents an advance in our knowledge on the regulation of oil accumulation in oil fruit.

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References

Ghosal A, Banas A, Stahl U, Dahlqvist A, Lindqvist Y, Stymne S . Saccharomyces cerevisiae phospholipid:diacylglycerol acyl transferase (PDAT) devoid of its membrane anchor region is a soluble and active enzyme retaining its substrate specificities. Biochim Biophys Acta. 2007; 1771(12):1457-63. DOI: 10.1016/j.bbalip.2007.10.007. View

Hernandez M, Mancha M, Martinez-Rivas J . Molecular cloning and characterization of genes encoding two microsomal oleate desaturases (FAD2) from olive. Phytochemistry. 2005; 66(12):1417-26. DOI: 10.1016/j.phytochem.2005.04.004. View

Martinelle M, Holmquist M, Clausen I, Patkar S, Svendsen A, Hult K . The role of Glu87 and Trp89 in the lid of Humicola lanuginosa lipase. Protein Eng. 1996; 9(6):519-24. DOI: 10.1093/protein/9.6.519. View

Aznar-Moreno J, Durrett T . Simultaneous Targeting of Multiple Gene Homeologs to Alter Seed Oil Production in Camelina sativa. Plant Cell Physiol. 2017; 58(7):1260-1267. DOI: 10.1093/pcp/pcx058. View

Feng Y, Zhang Y, Ding W, Wu P, Cao X, Xue S . Expanding of Phospholipid:Diacylglycerol AcylTransferase (PDAT) from Saccharomyces cerevisiae as Multifunctional Biocatalyst with Broad Acyl Donor/Acceptor Selectivity. Appl Biochem Biotechnol. 2019; 188(3):824-835. DOI: 10.1007/s12010-019-02954-x. View

Goncalves A, Silva E, Brito C, Martins S, Pinto L, Dinis L . Olive tree physiology and chemical composition of fruits are modulated by different deficit irrigation strategies. J Sci Food Agric. 2019; 100(2):682-694. DOI: 10.1002/jsfa.10064. View

Kim H, Lee K, Go Y, Jung J, Suh M, Kim J . Endoplasmic reticulum-located PDAT1-2 from castor bean enhances hydroxy fatty acid accumulation in transgenic plants. Plant Cell Physiol. 2011; 52(6):983-93. DOI: 10.1093/pcp/pcr051. View

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

Bates P . Understanding the control of acyl flux through the lipid metabolic network of plant oil biosynthesis. Biochim Biophys Acta. 2016; 1861(9 Pt B):1214-1225. DOI: 10.1016/j.bbalip.2016.03.021. View

10.

Demski K, Losiewska A, Jasieniecka-Gazarkiewicz K, Klinska S, Banas A . Phospholipid:Diacylglycerol Acyltransferase1 Overexpression Delays Senescence and Enhances Post-heat and Cold Exposure Fitness. Front Plant Sci. 2020; 11:611897. PMC: 7767865. DOI: 10.3389/fpls.2020.611897. View

11.

Mhaske V, Beldjilali K, Ohlrogge J, Pollard M . Isolation and characterization of an Arabidopsis thaliana knockout line for phospholipid: diacylglycerol transacylase gene (At5g13640). Plant Physiol Biochem. 2005; 43(4):413-7. DOI: 10.1016/j.plaphy.2005.01.013. View

12.

Yang Y, Benning C . Functions of triacylglycerols during plant development and stress. Curr Opin Biotechnol. 2017; 49:191-198. DOI: 10.1016/j.copbio.2017.09.003. View

13.

Weselake R, Taylor D, Rahman M, Shah S, Laroche A, McVetty P . Increasing the flow of carbon into seed oil. Biotechnol Adv. 2009; 27(6):866-878. DOI: 10.1016/j.biotechadv.2009.07.001. View

14.

Stahl U, Carlsson A, Lenman M, Dahlqvist A, Huang B, Banas W . Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis. Plant Physiol. 2004; 135(3):1324-35. PMC: 519051. DOI: 10.1104/pp.104.044354. View

15.

Hernandez M, Sicardo M, Alfonso M, Martinez-Rivas J . Transcriptional Regulation of Stearoyl-Acyl Carrier Protein Desaturase Genes in Response to Abiotic Stresses Leads to Changes in the Unsaturated Fatty Acids Composition of Olive Mesocarp. Front Plant Sci. 2019; 10:251. PMC: 6411816. DOI: 10.3389/fpls.2019.00251. View

16.

Peelman F, Verschelde J, Vanloo B, Ampe C, Labeur C, Tavernier J . Effects of natural mutations in lecithin:cholesterol acyltransferase on the enzyme structure and activity. J Lipid Res. 1998; 40(1):59-69. View

17.

Padilla M, Hernandez M, Sanz C, Martinez-Rivas J . Stress-dependent regulation of 13-lipoxygenases and 13-hydroperoxide lyase in olive fruit mesocarp. Phytochemistry. 2014; 102:80-8. DOI: 10.1016/j.phytochem.2014.01.024. View

18.

Schrag J, Cygler M . Lipases and alpha/beta hydrolase fold. Methods Enzymol. 1997; 284:85-107. DOI: 10.1016/s0076-6879(97)84006-2. View

19.

Hernandez M, Padilla M, Sicardo M, Mancha M, Martinez-Rivas J . Effect of different environmental stresses on the expression of oleate desaturase genes and fatty acid composition in olive fruit. Phytochemistry. 2011; 72(2-3):178-87. DOI: 10.1016/j.phytochem.2010.11.026. View

20.

Hernandez M, Padilla M, Mancha M, Martinez-Rivas J . Expression analysis identifies FAD2-2 as the olive oleate desaturase gene mainly responsible for the linoleic acid content in virgin olive oil. J Agric Food Chem. 2009; 57(14):6199-206. DOI: 10.1021/jf900678z. View