Genomic Cloning, Characterization, and Multilineage Growth-promoting Activity of Human Granulocyte-macrophage Colony-stimulating Factor

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 May 1

PMID 3486413

Citations 22

Authors

K Kaushansky

P J OHara

K Berkner

G M Segal

F S Hagen

J W Adamson

Affiliations

Soon will be listed here.

Abstract

Through the use of long single-sequence oligonucleotide probes, the complete gene for human granulocyte-macrophage colony-stimulating factor (hGM-CSF) has been cloned from a human genomic library. The gene is 2.5 kilobases in length, contains three introns, and is present as a single copy in the human genome. When subcloned into the mammalian expression vector pD3, the gene directs the synthesis of authentic hGM-CSF. In addition to its stimulation of in vitro granulopoiesis and monopoiesis, recombinant hGM-CSF stimulates in vitro erythropoiesis and megakaryopoiesis.

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