Siderophore-mediated Zinc Acquisition Enhances Enterobacterial Colonization of the Inflamed Gut

Overview

Journal Nat Commun

Specialty Biology

Date 2021 Dec 2

PMID 34853318

Citations 32

Authors

Judith Behnsen

Hui Zhi

Allegra T Aron

Vivekanandan Subramanian

William Santus

Michael H Lee

Romana R Gerner

Daniel Petras

Janet Z Liu

Keith D Green

Sarah L Price

Jose Camacho

Hannah Hillman

Joshua Tjokrosurjo

Nicola P Montaldo

Evelyn M Hoover

Sean Treacy-Abarca

Benjamin A Gilston

Eric P Skaar

Walter J Chazin

Sylvie Garneau-Tsodikova

Matthew B Lawrenz

Robert D Perry

Sean-Paul Nuccio

Pieter C Dorrestein

Manuela Raffatellu

Affiliations

Soon will be listed here.

Abstract

Zinc is an essential cofactor for bacterial metabolism, and many Enterobacteriaceae express the zinc transporters ZnuABC and ZupT to acquire this metal in the host. However, the probiotic bacterium Escherichia coli Nissle 1917 (or "Nissle") exhibits appreciable growth in zinc-limited media even when these transporters are deleted. Here, we show that Nissle utilizes the siderophore yersiniabactin as a zincophore, enabling Nissle to grow in zinc-limited media, to tolerate calprotectin-mediated zinc sequestration, and to thrive in the inflamed gut. We also show that yersiniabactin's affinity for iron or zinc changes in a pH-dependent manner, with increased relative zinc binding as the pH increases. Thus, our results indicate that siderophore metal affinity can be influenced by the local environment and reveal a mechanism of zinc acquisition available to commensal and pathogenic Enterobacteriaceae.

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