One-Step Microfluidic Fabrication of Multi-Responsive Liposomes for Targeted Delivery of Doxorubicin Synergism with Photothermal Effect

Overview

Journal Int J Nanomedicine

Publisher Dove Medical Press

Specialty Biotechnology

Date 2021 Dec 1

PMID 34848958

Citations 11

Authors

Songwei Lv

Ran Jing

Xiaowu Liu

Honglei Shi

Yunfeng Shi

Xugang Wang

Xiubo Zhao

Kai Cao

Zhong Lv

Affiliations

Soon will be listed here.

Abstract

Introduction: Cancer of the bladder is one of the most common and life-threatening. Compared with traditional delivery methods, intravesical administration reduces the amount of drugs required, increases the amount of drugs reaching the lesion site, and minimizes systemic exposure to therapeutic agents. To overcome the limitations of urinary voiding, low urothelium permeability, and intermittent catheterization for large dilution and irrigation of drugs in the bladder, magnetic and photothermal-responsive folate receptor-targeted thermal liposomes (FA-TMLs) were designed for the targeted delivery of doxorubicin (DOX) to bladder cancer cells.

Methods: Through a microfluidic mixer chip, the magnetic nanoparticles (MNPs), gold nanorods (GNRs) and DOX were encapsulated in folate-modified thermosensitive liposomes to form FA-TMLs@MNPs-GNRs-DOX. DLS, TEM, DSC, and magnetic hysteresis loop were used to characterize the construction of FA-TMLs@MNPs-GNRs-DOX.

Results: FA-TMLs@MNPs-GNRs-DOX had a size of about 230 nm and exhibited superparamagnetic properties with the saturation magnetization of 20 emu/g. The DOX loading capacity was as high as 0.57 mg/mL. Additionally, drug release of the FA-TMLs@MNPs-GNRs-DOX could be controlled by temperature change through the photothermal effect. A 980 nm laser beam was selectively irradiated on the FA-TMLs@MNPs-GNRs-DOX to trigger the structural changes of the FA-TMLs, and an average of 95% of the drug was released after 3 hours. The results of cell uptake experiments reveal indicated that FA-TMLs@MNPs-GNRs-DOX were able to specifically bind folate-receptor-positive cells and exhibited toxicity to bladder tumor cells.

Conclusion: The present results suggest FA-TMLs@MNPs-GNRs-DOX have a promising multifunctional response and can act as an ideal multifunctional drug delivery system (DDS) for the treatment of bladder tumors.

Citing Articles

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Zeng X Int J Nanomedicine. 2025; 20:1635-1636.

PMID: 39925676 PMC: 11807347. DOI: 10.2147/IJN.S518304.

Applications of liposomes and lipid nanoparticles in cancer therapy: current advances and prospects.

Cheng Z, Huang H, Yin M, Liu H Exp Hematol Oncol. 2025; 14(1):11.

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Microfluidic Synthesis of miR-200c-3p Lipid Nanoparticles: Targeting ZEB2 to Alleviate Chondrocyte Damage in Osteoarthritis.

Zheng D, Chen T, Yang K, Yin G, Chen Y, Gui J Int J Nanomedicine. 2025; 20():505-521.

PMID: 39830158 PMC: 11742371. DOI: 10.2147/IJN.S491711.

Intertumoral and intratumoral barriers as approaches for drug delivery and theranostics to solid tumors using stimuli-responsive materials.

Chenab K, Malektaj H, Nadinlooie A, Mohammadi S, Zamani-Meymian M Mikrochim Acta. 2024; 191(9):541.

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Polystyrene nanoplastics of different particle sizes regulate the polarization of pro-inflammatory macrophages.

Jiang W, Liu Y, Wu Y, Zhang L, Zhang B, Zhou S Sci Rep. 2024; 14(1):16329.

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