Oxidation Sensitizes TRPV2 to Chemical and Heat Stimuli, but Not Mechanical Stimulation

Overview

Journal Biochem Biophys Rep

Specialty Biochemistry

Date 2021 Nov 29

PMID 34841092

Citations 3

Authors

Mai Oda

Yuichiro Fujiwara

Yasuki Ishizaki

Koji Shibasaki

Affiliations

Soon will be listed here.

Abstract

The transient receptor potential vanilloid 2 (TRPV2) ion channel is activated by a chemical ligand (2-aminoethoxydiphenyl borate; 2-APB), noxious heat and mechanical stimulation. In a heterologous mammalian cell expression system, the oxidant chloramine T (ChT) sensitizes TRPV2 activation in response to 2-APB and heat by oxidation of methionine residues at positions 528 and 607 in rat TRPV2. Here, we used a oocyte expression system to determine whether ChT-mediated oxidation can also sensitize TRPV2 to mechanical stimulation. In this system, we confirmed that ChT sensitized TRPV2 activation in response to 2-APB and heat, but we detected no sensitization to mechanical stimulation. This result suggests that the activation mechanism of TRPV2 by a chemical ligand and heat differs from that for mechanical stimulation. Further, we demonstrated that two-electrode voltage clamp recording in the oocyte expression system is an excellent format for high throughput analysis of oxidization of redox-sensitive TRP channels.

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