Voltammetric Sensor Based on Bimetallic Nanocomposite for Determination of Favipiravir As an Antiviral Drug

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2021 Nov 27

PMID 34837114

Citations 22

Authors

Mohammad Mehmandoust

Yasamin Khoshnavaz

Mustafa Tuzen

Nevin Erk

Affiliations

Soon will be listed here.

Abstract

A novel and sensitive voltammetric nanosensor was developed for the first time for trace level monitoring of favipiravir based on gold/silver core-shell nanoparticles (Au@Ag CSNPs) with conductive polymer poly (3,4-ethylene dioxythiophene) polystyrene sulfonate (PEDOT:PSS) and functionalized multi carbon nanotubes (F-MWCNTs) on a glassy carbon electrode (GCE). The formation of Au@Ag CSNPs/PEDOT:PSS/F-MWCNT composite was confirmed by various analytical techniques, including X-ray diffraction (XRD), ultraviolet-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDX), and field-emission scanning electron microscopy (SEM). Under the optimized conditions and at a typical working potential of + 1.23 V (vs. Ag/AgCl), the Au@Ag CSNPs/PEDOT:PSS/F-MWCNT/GCE revealed linear quantitative ranges from 0.005 to 0.009 and 0.009 to 1.95 µM with a limit of detection 0.46 nM (S/N = 3) with acceptable relative standard deviations (1.1-4.9 %) for pharmaceutical formulations, urine, and human plasma samples without applying any sample pretreatment (1.12-4.93%). The interference effect of antiviral drugs, biological compounds, and amino acids was negligible, and the sensing system demonstrated outstanding reproducibility, repeatability, stability, and reusability. The findings revealed that this assay strategy has promising applications in diagnosing FAV in clinical samples, which could be attributed to the large surface area on active sites and high conductivity of bimetallic nanocomposite.

Citing Articles

Eco-sustainable chromatographic method for the determination of favipiravir and nitazoxanide for COVID-19: application to human plasma.

Ahmed A, Abdelrahman M, Edrees F BMC Chem. 2025; 19(1):11.

PMID: 39789629 PMC: 11714856. DOI: 10.1186/s13065-024-01364-3.

Pharmacogenomic Studies of Antiviral Drug Favipiravir.

Shumyantseva V, Bulko T, Chistov A, Kolesanova E, Agafonova L Pharmaceutics. 2024; 16(4).

PMID: 38675164 PMC: 11053860. DOI: 10.3390/pharmaceutics16040503.

Green chemometric-assisted UV-spectrophotometric methods for the determination of favipiravir, cefixime and moxifloxacin hydrochloride as an effective therapeutic combination for COVID-19; application in pharmaceutical form and spiked human plasma.

Madbouly E, El-Shanawani A, El-Adl S, Abdelkhalek A BMC Chem. 2024; 18(1):65.

PMID: 38575973 PMC: 10996251. DOI: 10.1186/s13065-024-01168-5.

A solvent-free HPLC method for the simultaneous determination of Favipiravir and its hydrolytic degradation product.

Sharaf Y, Abd El-Fattah M, El-Sayed H, Hassan S Sci Rep. 2023; 13(1):18512.

PMID: 37898682 PMC: 10613211. DOI: 10.1038/s41598-023-45618-x.

Spectrophotometric determination of favipiravir in presence of its acid hydrolysis product.

Sharaf Y, Abd El-Fattah M, El-Sayed H, Hegazy M BMC Chem. 2023; 17(1):129.

PMID: 37777796 PMC: 10542695. DOI: 10.1186/s13065-023-01046-6.

References

Shannon A, Selisko B, Le N, Huchting J, Touret F, Piorkowski G . Rapid incorporation of Favipiravir by the fast and permissive viral RNA polymerase complex results in SARS-CoV-2 lethal mutagenesis. Nat Commun. 2020; 11(1):4682. PMC: 7499305. DOI: 10.1038/s41467-020-18463-z. View

Prakash S, Chakrabarty T, Singh A, Shahi V . Polymer thin films embedded with metal nanoparticles for electrochemical biosensors applications. Biosens Bioelectron. 2012; 41:43-53. DOI: 10.1016/j.bios.2012.09.031. View

Clarke J, Majeed A, Beaney T . Measuring the impact of covid-19. BMJ. 2021; 373:n1239. DOI: 10.1136/bmj.n1239. View

Karaman C, Karaman O, Atar N, Yola M . Electrochemical immunosensor development based on core-shell high-crystalline graphitic carbon nitride@carbon dots and CdZnS/d-TiCT MXene composite for heart-type fatty acid-binding protein detection. Mikrochim Acta. 2021; 188(6):182. DOI: 10.1007/s00604-021-04838-6. View

Ensafi A, Dadkhah-Tehrani S, Karimi-Maleh H . A voltammetric sensor for the simultaneous determination of L-cysteine and tryptophan using a p-aminophenol-multiwall carbon nanotube paste electrode. Anal Sci. 2011; 27(4):409. DOI: 10.2116/analsci.27.409. View

Furuta Y, Gowen B, Takahashi K, Shiraki K, Smee D, Barnard D . Favipiravir (T-705), a novel viral RNA polymerase inhibitor. Antiviral Res. 2013; 100(2):446-54. PMC: 3880838. DOI: 10.1016/j.antiviral.2013.09.015. View

Cao X . COVID-19: immunopathology and its implications for therapy. Nat Rev Immunol. 2020; 20(5):269-270. PMC: 7143200. DOI: 10.1038/s41577-020-0308-3. View

Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y . Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020; 395(10223):497-506. PMC: 7159299. DOI: 10.1016/S0140-6736(20)30183-5. View

Morsy M, Nouman E, Abdallah Y, Zainelabdeen M, Darwish M, Hassan A . A novel LC-MS/MS method for determination of the potential antiviral candidate favipiravir for the emergency treatment of SARS-CoV-2 virus in human plasma: Application to a bioequivalence study in Egyptian human volunteers. J Pharm Biomed Anal. 2021; 199:114057. PMC: 8015396. DOI: 10.1016/j.jpba.2021.114057. View

10.

Hale B, Albrecht R, Garcia-Sastre A . Innate immune evasion strategies of influenza viruses. Future Microbiol. 2009; 5(1):23-41. PMC: 2820251. DOI: 10.2217/fmb.09.108. View

11.

Bulduk I . Comparison of HPLC and UV Spectrophotometric Methods for Quantification of Favipiravir in Pharmaceutical Formulations. Iran J Pharm Res. 2021; 20(3):57-65. PMC: 8653657. DOI: 10.22037/ijpr.2020.114199.14725. View

12.

Allahverdiyeva S, Yunusoglu O, Yardim Y, Senturk Z . First electrochemical evaluation of favipiravir used as an antiviral option in the treatment of COVID-19: A study of its enhanced voltammetric determination in cationic surfactant media using a boron-doped diamond electrode. Anal Chim Acta. 2021; 1159:338418. PMC: 7971419. DOI: 10.1016/j.aca.2021.338418. View

13.

Habler K, Brugel M, Teupser D, Liebchen U, Scharf C, Schonermarck U . Simultaneous quantification of seven repurposed COVID-19 drugs remdesivir (plus metabolite GS-441524), chloroquine, hydroxychloroquine, lopinavir, ritonavir, favipiravir and azithromycin by a two-dimensional isotope dilution LC-MS/MS method in human.... J Pharm Biomed Anal. 2021; 196:113935. PMC: 7843035. DOI: 10.1016/j.jpba.2021.113935. View

14.

Delang L, Abdelnabi R, Neyts J . Favipiravir as a potential countermeasure against neglected and emerging RNA viruses. Antiviral Res. 2018; 153:85-94. DOI: 10.1016/j.antiviral.2018.03.003. View

15.

Karimi-Maleh H, Orooji Y, Karimi F, Alizadeh M, Baghayeri M, Rouhi J . A critical review on the use of potentiometric based biosensors for biomarkers detection. Biosens Bioelectron. 2021; 184:113252. DOI: 10.1016/j.bios.2021.113252. View

16.

Shankar S, Rai A, Ahmad A, Sastry M . Rapid synthesis of Au, Ag, and bimetallic Au core-Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci. 2004; 275(2):496-502. DOI: 10.1016/j.jcis.2004.03.003. View

17.

Mohamed M, Eldin G, Ismail S, Zine N, Elaissari A, Jaffrezic-Renault N . Innovative electrochemical sensor for the precise determination of the new antiviral COVID-19 treatment Favipiravir in the presence of coadministered drugs. J Electroanal Chem (Lausanne). 2021; 895:115422. PMC: 8161794. DOI: 10.1016/j.jelechem.2021.115422. View

18.

De Clercq E . New Nucleoside Analogues for the Treatment of Hemorrhagic Fever Virus Infections. Chem Asian J. 2019; 14(22):3962-3968. PMC: 7159701. DOI: 10.1002/asia.201900841. View

19.

Xu Z, Shi L, Wang Y, Zhang J, Huang L, Zhang C . Pathological findings of COVID-19 associated with acute respiratory distress syndrome. Lancet Respir Med. 2020; 8(4):420-422. PMC: 7164771. DOI: 10.1016/S2213-2600(20)30076-X. View

20.

Sanghavi B, Srivastava A . Simultaneous voltammetric determination of acetaminophen and tramadol using Dowex50wx2 and gold nanoparticles modified glassy carbon paste electrode. Anal Chim Acta. 2011; 706(2):246-54. DOI: 10.1016/j.aca.2011.08.040. View