Thinking Quantitatively of RNA-Based Information Transfer Via Extracellular Vesicles: Lessons to Learn for the Design of RNA-Loaded EVs

Overview

Journal Pharmaceutics

Publisher MDPI

Date 2021 Nov 27

PMID 34834346

Citations 14

Authors

Max Piffoux

Jeanne Volatron

Amanda K A Silva

Florence Gazeau

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are 50-1000 nm vesicles secreted by virtually any cell type in the body. They are expected to transfer information from one cell or tissue to another in a short- or long-distance way. RNA-based transfer of information via EVs at long distances is an interesting well-worn hypothesis which is ~15 years old. We review from a quantitative point of view the different facets of this hypothesis, ranging from natural RNA loading in EVs, EV pharmacokinetic modeling, EV targeting, endosomal escape and RNA delivery efficiency. Despite the unique intracellular delivery properties endowed by EVs, we show that the transfer of RNA naturally present in EVs might be limited in a physiological context and discuss the lessons we can learn from this example to design efficient RNA-loaded engineered EVs for biotherapies. We also discuss other potential EV mediated information transfer mechanisms, among which are ligand-receptor mechanisms.

Citing Articles

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Tumor Cell Communications as Promising Supramolecular Targets for Cancer Chemotherapy: A Possible Strategy.

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