Cloning, Expression, and Tobacco Overexpression Analyses of a /-like () Gene from

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2021 Nov 27

PMID 34828354

Authors

Zhanghui Zeng

Si Chen

Mingrui Xu

Min Wang

Zhehao Chen

Lilin Wang

Jiliang Pang

Affiliations

Soon will be listed here.

Abstract

(), a B-class MADS-box gene, is involved in floral organ determination but has rarely been studied in , which is a very popular ornamental tree species in China. Here, the full-length cDNA of a homologous gene (named ) was cloned from a flower bud of using the RACE technique. The has a 645 bp open reading frame, encoding 214 amino acids. Similar to other PI/GLO proteins, OfGLO1 has two conserved domains, MADS MEF2-like and K-box, and a 16-amino-acid PI motif in the C terminal region. Our phylogeny analysis classified OfGLO1 as a PI-type member of the B-class MADS-box gene family. The qRT-PCR assay showed that the expression of in was continuously upregulated from the tight bud stage to the full flowering stage but barely expressed in the pistils, sepals, and non-floral organs, such as root, leaf, and stem. The genetic effect of was assayed by ectopic expression in tobacco plants. Compared with the wild-type, transformants showed reduced plant size, earlier flowering, shorter stamens, and lower seed setting rates. Furthermore, some stamens were changed into petal-like structures. These findings indicate that plays an important role in the regulation of flower development. This study improved our understanding of class B gene function in woody plants.

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