MiR-301a-5p Regulates During Chicken Spermatogenesis

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2021 Nov 27

PMID 34828300

Citations 2

Authors

Qixin Guo

Yong Jiang

Hao Bai

Guohong Chen

Guobin Chang

Affiliations

Soon will be listed here.

Abstract

The process of spermatogenesis is complex and systemic, requiring the cooperation of many regulators. However, little is known about how micro RNAs (miRNAs) regulate spermatogenesis in poultry. In this study, we investigated key miRNAs and their target genes that are involved in spermatogenesis in chickens. Next-generation sequencing was conducted to determine miRNA expression profiles in five cell types: primordial germ cells (PGCs), spermatogonial stem cells (SSCs), spermatogonia (Spa), and chicken sperm. Next, we analyzed and identified several key miRNAs that regulate spermatogenesis in the four germline cell miRNA profiles. Among the enriched miRNAs, miRNA-301a-5p was the key miRNA in PGCs, SSCs, and Spa. Through reverse transcription quantitative PCR (RT-qPCR), dual-luciferase, and miRNA salience, we confirmed that miR-301a-5p binds to transforming growth factor-beta 2 () and is involved in the transforming growth factor-beta (TGF-β) signaling pathway and germ cell development. To the best of our knowledge, this is the first demonstration of miR-301a-5p involvement in spermatogenesis by direct binding to , a key gene in the TGF-β signaling pathway. This finding contributes to the insights into the molecular mechanism through which miRNAs regulate germline cell differentiation and spermatogenesis in chickens.

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References

Moraveji S, Esfandiari F, Taleahmad S, Nikeghbalian S, Sayahpour F, Masoudi N . Suppression of transforming growth factor-beta signaling enhances spermatogonial proliferation and spermatogenesis recovery following chemotherapy. Hum Reprod. 2019; 34(12):2430-2442. DOI: 10.1093/humrep/dez196. View

Beyret E, Liu N, Lin H . piRNA biogenesis during adult spermatogenesis in mice is independent of the ping-pong mechanism. Cell Res. 2012; 22(10):1429-39. PMC: 3463270. DOI: 10.1038/cr.2012.120. View

Wang X, Liu Q, Xu S, Xiao Y, Wang Y, Feng C . Transcriptome Dynamics During Turbot Spermatogenesis Predicting the Potential Key Genes Regulating Male Germ Cell Proliferation and Maturation. Sci Rep. 2018; 8(1):15825. PMC: 6202422. DOI: 10.1038/s41598-018-34149-5. View

Alves M, Arruda R, De Bem T, Florez-Rodriguez S, de Sa Filho M, Belleannee C . Sperm-borne miR-216b modulates cell proliferation during early embryo development via K-RAS. Sci Rep. 2019; 9(1):10358. PMC: 6637201. DOI: 10.1038/s41598-019-46775-8. View

Guo W, Xie B, Xiong S, Liang X, Gui J, Mei J . miR-34a Regulates Sperm Motility in Zebrafish. Int J Mol Sci. 2017; 18(12). PMC: 5751278. DOI: 10.3390/ijms18122676. View

Griswold M . Spermatogenesis: The Commitment to Meiosis. Physiol Rev. 2015; 96(1):1-17. PMC: 4698398. DOI: 10.1152/physrev.00013.2015. View

Gautier C, Levacher C, Saez J, Habert R . Transforming growth factor beta1 inhibits steroidogenesis in dispersed fetal testicular cells in culture. Mol Cell Endocrinol. 1997; 131(1):21-30. DOI: 10.1016/s0303-7207(97)00087-7. View

Tong M, Mitchell D, McGowan S, Evanoff R, Griswold M . Two miRNA clusters, Mir-17-92 (Mirc1) and Mir-106b-25 (Mirc3), are involved in the regulation of spermatogonial differentiation in mice. Biol Reprod. 2011; 86(3):72. PMC: 3316268. DOI: 10.1095/biolreprod.111.096313. View

La H, Hobbs R . Mechanisms regulating mammalian spermatogenesis and fertility recovery following germ cell depletion. Cell Mol Life Sci. 2019; 76(20):4071-4102. PMC: 11105665. DOI: 10.1007/s00018-019-03201-6. View

10.

OBrien J, Hayder H, Zayed Y, Peng C . Overview of MicroRNA Biogenesis, Mechanisms of Actions, and Circulation. Front Endocrinol (Lausanne). 2018; 9:402. PMC: 6085463. DOI: 10.3389/fendo.2018.00402. View

11.

He Z, Kokkinaki M, Pant D, Gallicano G, Dym M . Small RNA molecules in the regulation of spermatogenesis. Reproduction. 2009; 137(6):901-11. DOI: 10.1530/REP-08-0494. View

12.

Kotaja N . MicroRNAs and spermatogenesis. Fertil Steril. 2014; 101(6):1552-62. DOI: 10.1016/j.fertnstert.2014.04.025. View

13.

Wang L, Xu C . Role of microRNAs in mammalian spermatogenesis and testicular germ cell tumors. Reproduction. 2014; 149(3):R127-37. DOI: 10.1530/REP-14-0239. View

14.

Ni F, Hao S, Yang W . Multiple signaling pathways in Sertoli cells: recent findings in spermatogenesis. Cell Death Dis. 2019; 10(8):541. PMC: 6637205. DOI: 10.1038/s41419-019-1782-z. View

15.

Dexheimer P, Cochella L . MicroRNAs: From Mechanism to Organism. Front Cell Dev Biol. 2020; 8:409. PMC: 7283388. DOI: 10.3389/fcell.2020.00409. View

16.

Li M, Mruk D, Cheng C . Mitogen-activated protein kinases in male reproductive function. Trends Mol Med. 2009; 15(4):159-68. PMC: 2804913. DOI: 10.1016/j.molmed.2009.02.002. View

17.

Shi Y, Massague J . Mechanisms of TGF-beta signaling from cell membrane to the nucleus. Cell. 2003; 113(6):685-700. DOI: 10.1016/s0092-8674(03)00432-x. View

18.

Kleene K . Connecting cis-elements and trans-factors with mechanisms of developmental regulation of mRNA translation in meiotic and haploid mammalian spermatogenic cells. Reproduction. 2013; 146(1):R1-19. DOI: 10.1530/REP-12-0362. View

19.

Moreno S, Attali M, Allemand I, Messiaen S, Fouchet P, Coffigny H . TGFbeta signaling in male germ cells regulates gonocyte quiescence and fertility in mice. Dev Biol. 2010; 342(1):74-84. DOI: 10.1016/j.ydbio.2010.03.007. View

20.

Meikar O, Da Ros M, Kotaja N . Epigenetic regulation of male germ cell differentiation. Subcell Biochem. 2012; 61:119-38. DOI: 10.1007/978-94-007-4525-4_6. View