Phosphorothioate-DNA Bacterial Diet Reduces the ROS Levels in C. Elegans While Improving Locomotion and Longevity

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Nov 26

PMID 34824369

Citations 3

Authors

Qiang Huang

Ruohan Li

Tao Yi

Fengsong Cong

Dayong Wang

Zixin Deng

Yi-Lei Zhao

Affiliations

Soon will be listed here.

Abstract

DNA phosphorothioation (PT) is widely distributed in the human gut microbiome. In this work, PT-diet effect on nematodes was studied with PT-bioengineering bacteria. We found that the ROS level decreased by about 20-50% and the age-related lipofuscin accumulation was reduced by 15-25%. Moreover, the PT-feeding worms were more active at all life periods, and more resistant to acute stressors. Intriguingly, their lifespans were prolonged by ~21.7%. Comparative RNA-seq analysis indicated that many gene expressions were dramatically regulated by PT-diet, such as cysteine-rich protein (scl-11/12/13), sulfur-related enzyme (cpr-2), longevity gene (jnk-1) and stress response (sod-3/5, gps-5/6, gst-18/20, hsp-12.8). Both the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis suggested that neuroactivity pathways were upregulated, while phosphoryl transfer and DNA-repair pathways were down-regulated in good-appetite young worms. The findings pave the way for pro-longevity of multicellular organisms by PT-bacterial interference.

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