Characteristics of Two Crustins from in Hydrothermal Vents

Overview

Journal Mar Drugs

Publisher MDPI

Specialties Biology
Pharmacology

Date 2021 Nov 25

PMID 34822471

Citations 2

Authors

Lu-Lu Guo

Shao-Lu Wang

Fang-Chao Zhu

Feng Xue

Li-Sheng He

Affiliations

Soon will be listed here.

Abstract

Crustins are widely distributed among different crustacean groups. They are characterized by a whey acidic protein (WAP) domain, and most examined Crustins show activity against Gram-positive bacteria. This study reports two Crustins, Al-crus 3 and Al-crus 7, from hydrothermal vent shrimp, . Al-crus 3 and Al-crus 7 belong to Crustin Type IIa, with a similarity of about 51% at amino acid level. Antibacterial assays showed that Al-crus 3 mainly displayed activity against Gram-positive bacteria with MIC values of 10-25 μM. However, Al-crus 7 not only displayed activity against Gram-positive bacteria but also against Gram-negative bacteria Imipenem-resistant , in a sensitive manner. Notably, in the effective antibacterial spectrum, Methicillin-sensitive , (ESBLs) and Imipenem-resistant were drug-resistant pathogens. Narrowing down the sequence to the WAP domain, Al-crusWAP 3 and Al-crusWAP 7 demonstrated antibacterial activities but were weak. Additionally, the effects on bacteria did not significantly change after they were maintained at room temperature for 48 h. This indicated that Al-crus 3 and Al-crus 7 were relatively stable and convenient for transportation. Altogether, this study reported two new Crustins with specific characteristics. In particular, Al-crus 7 inhibited Gram-negative imipenem-resistant .

Citing Articles

A New Crustin Gene Homolog SpCrus8 Identified in Exerting Protection Through Opsonization and Immunomodulation.

Jiang M, Chen R, Chen F, Zhu X, Wang K Front Immunol. 2022; 13:946227.

PMID: 35874773 PMC: 9305162. DOI: 10.3389/fimmu.2022.946227.

A Type Ib Crustin from Deep-Sea Shrimp Possesses Antimicrobial and Immunomodulatory Activity.

Wang Y, Sun L Int J Mol Sci. 2022; 23(12).

PMID: 35742887 PMC: 9223358. DOI: 10.3390/ijms23126444.

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