Single-molecule Manipulation of Macromolecules on GUV or SUV Membranes Using Optical Tweezers

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2021 Nov 23

PMID 34813728

Citations 8

Authors

Yukun Wang

Avinash Kumar

Huaizhou Jin

Yongli Zhang

Affiliations

Soon will be listed here.

Abstract

Despite their wide applications in soluble macromolecules, optical tweezers have rarely been used to characterize the dynamics of membrane proteins, mainly due to the lack of model membranes compatible with optical trapping. Here, we examined optical trapping and mechanical properties of two potential model membranes, giant and small unilamellar vesicles (GUVs and SUVs, respectively) for studies of membrane protein dynamics. We found that optical tweezers can stably trap GUVs containing iodixanol with controlled membrane tension. The trapped GUVs with high membrane tension can serve as a force sensor to accurately detect reversible folding of a DNA hairpin or membrane binding of synaptotagmin-1 C2AB domain attached to the GUV. We also observed that SUVs are rigid enough to resist large pulling forces and are suitable for detecting protein conformational changes induced by force. Our methodologies may facilitate single-molecule manipulation studies of membrane proteins using optical tweezers.

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