ITRAQ-based Proteomic Analysis of Differentially Expressed Proteins in Sera of Seronegative and Seropositive Rheumatoid Arthritis Patients

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2021 Nov 23

PMID 34812532

Citations 2

Authors

Yujue He

Junyu Lin

Jifeng Tang

Ziqing Yu

Qishui Ou

Jinpiao Lin

Affiliations

Soon will be listed here.

Abstract

Objective: The diagnosis of seronegative rheumatoid arthritis (SNRA) is often difficult due to the unavailability of reliable laboratory markers. The aim of this study was to identify differentially expressed proteins in sera of SNRA, seropositive RA (SPRA), and healthy donors (HD).

Methods: A total of 32 seropositive RA patients, 32 SNRA patients, and 35 HD were enrolled in our study. Differentially expressed proteins between 3 groups were identified via isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic analysis, and an ELISA test was used for the validation test. Correlation analysis was conducted by GraphPad Prism.

Results: Using iTRAQ quantitative proteomics, we identified 14 proteins were significantly different between SPRA and SNRA, including 4 upregulated proteins and 10 downregulated proteins. Four differentially expressed proteins were validated by ELISA test, and the results showed that SAA1 protein was significantly higher in SPRA and SNRA patients compared with HD, and PSME1 was elevated in SPRA patients. What's more, SAA1 was increased in the anti-CCP or RF high-level group in RA patients, and PSME1 was increased in the RF high-level group. Alternatively, SAA1 was positively correlated with inflammation indicators in RA patients, while PSME1 showed no correlation with inflammation indicators.

Conclusions: iTRAQ proteomic approaches revealed variations in serum protein composition among SPRA patients, SNRA patients, and HD and provided new idea for advanced diagnostic methods and precision treatment of RA.

Citing Articles

The mechanism of the anti-inflammatory effect of Oldenlandia diffusa on arthritis model rats: a quantitative proteomic and network pharmacologic study.

Zhu H, Xiong X, Lu Y, Wu H, Zhang Z, Sun M Ann Transl Med. 2022; 10(20):1098.

PMID: 36388817 PMC: 9652507. DOI: 10.21037/atm-22-3678.

iTRAQ-based proteomic analysis of differentially expressed proteins in sera of seronegative and seropositive rheumatoid arthritis patients.

He Y, Lin J, Tang J, Yu Z, Ou Q, Lin J J Clin Lab Anal. 2021; 36(1):e24133.

PMID: 34812532 PMC: 8761432. DOI: 10.1002/jcla.24133.

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