Cell Sheet Transplantation Prevents Inflammatory Adhesions: A New Treatment for Adhesive Otitis Media

Overview

Journal Regen Ther

Date 2021 Nov 22

PMID 34805451

Citations 6

Authors

Kazuhisa Yamamoto

Tsunetaro Morino

Yoshiyuki Kasai

Shun Kikuchi

Manabu Komori

Masayuki Yamato

Hiromi Kojima

Affiliations

Soon will be listed here.

Abstract

Introduction: We developed a new treatment method that combines tympanoplasty with transplantation of autologous cultured nasal mucosal epithelial cell sheets to regenerate the mucosa of patients with adhesive otitis media, which has been difficult to treat effectively. We verified whether this procedure could be performed safely and measured its therapeutic efficacy.

Methods: Autologous nasal mucosal epithelial cell sheets were manufactured at a good manufacturing practice-compliant cell processing facility using autologous nasal mucosal tissue. We performed tympanoplasty and transplanted the cell sheets into the middle ear cavity in six patients with adhesive otitis media.

Results: The manufactured autologous cultured epithelial cell sheets met the predetermined quality standards and were successfully transplanted safely in all cases. Computed tomography findings after cell sheet transplantation showed that aeration in the tympanic cavity was maintained or restored in five of the six patients (83.3%). Four of the six (66.7%) patients had postoperative air-bone gap within 20 dB, which is considered a postoperative success in tympanoplasty for chronic middle ear disease.

Conclusions: The results of this clinical study suggest that tympanoplasty with cell sheet transplantation can be used to treat adhesive otitis media by reliably preventing re-adhesion of the tympanic membrane.

Citing Articles

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Engineering of MSCs sheet for the prevention of myocardial ischemia and for left ventricle remodeling.

Chang D, Yang X, Fan S, Fan T, Zhang M, Ono M Stem Cell Res Ther. 2023; 14(1):102.

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Analysis of the potential of human cultured nasal epithelial cell sheets to differentiate into airway epithelium.

Kasai Y, Morino T, Nakayama T, Yamamoto K, Kojima H FASEB Bioadv. 2023; 5(3):89-100.

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Transplantation of hybrid adipose-derived stem cell sheet with autologous peritoneum: An feasibility study.

Matsuo N, Ohki T, Aoyama S, Yamaguchi S, Itabashi M, Egawa H Heliyon. 2023; 9(2):e12992.

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Cell Sheet Technology as an Engineering-Based Approach to Bone Regeneration.

You Q, Lu M, Li Z, Zhou Y, Tu C Int J Nanomedicine. 2022; 17:6491-6511.

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References

Nishida K, Yamato M, Hayashida Y, Watanabe K, Yamamoto K, Adachi E . Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium. N Engl J Med. 2004; 351(12):1187-96. DOI: 10.1056/NEJMoa040455. View

Kanzaki M, Takagi R, Washio K, Kokubo M, Yamato M . Bio-artificial pleura using an autologous dermal fibroblast sheet. NPJ Regen Med. 2018; 2:26. PMC: 5677916. DOI: 10.1038/s41536-017-0031-2. View

Shudo Y, Miyagawa S, Nakatani S, Fukushima S, Sakaguchi T, Saito A . Myocardial layer-specific effect of myoblast cell-sheet implantation evaluated by tissue strain imaging. Circ J. 2013; 77(4):1063-72. DOI: 10.1253/circj.cj-12-0615. View

Hama T, Yamamoto K, Yaguchi Y, Murakami D, Sasaki H, Yamato M . Autologous human nasal epithelial cell sheet using temperature-responsive culture insert for transplantation after middle ear surgery. J Tissue Eng Regen Med. 2015; 11(4):1089-1096. DOI: 10.1002/term.2012. View

Yamato M, Utsumi M, Kushida A, Konno C, Kikuchi A, Okano T . Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. Tissue Eng. 2001; 7(4):473-80. DOI: 10.1089/10763270152436517. View

Yaguchi Y, Murakami D, Yamato M, Hama T, Yamamoto K, Kojima H . Middle ear mucosal regeneration with three-dimensionally tissue-engineered autologous middle ear cell sheets in rabbit model. J Tissue Eng Regen Med. 2013; 10(3):E188-94. DOI: 10.1002/term.1790. View

Yaguchi Y, Wada K, Uchimizu H, Tanaka Y, Kojima H, Moriyama H . Middle ear mucosa regeneration by grafting of artificial mucosa. Acta Otolaryngol. 2007; 127(10):1038-44. DOI: 10.1080/00016480701200285. View

Narita T, Shintani Y, Ikebe C, Kaneko M, Campbell N, Coppen S . The use of scaffold-free cell sheet technique to refine mesenchymal stromal cell-based therapy for heart failure. Mol Ther. 2013; 21(4):860-7. PMC: 3616531. DOI: 10.1038/mt.2013.9. View

Wada K, Tanaka Y, Kojima H, Inamatsu M, Yoshizato K, Moriyama H . In vitro reconstruction of a three-dimensional middle ear mucosal organ and its in vivo transplantation. Acta Otolaryngol. 2006; 126(8):801-10. DOI: 10.1080/00016480500507385. View

10.

Yamamoto K, Yamato M, Morino T, Sugiyama H, Takagi R, Yaguchi Y . Middle ear mucosal regeneration by tissue-engineered cell sheet transplantation. NPJ Regen Med. 2018; 2:6. PMC: 5665617. DOI: 10.1038/s41536-017-0010-7. View

11.

McGhee M, Dornhoffer J . The effect of gelfilm in the prevention of fibrosis in the middle ear of the animal model. Am J Otol. 1999; 20(6):712-6. View

12.

Sawa Y, Miyagawa S, Sakaguchi T, Fujita T, Matsuyama A, Saito A . Tissue engineered myoblast sheets improved cardiac function sufficiently to discontinue LVAS in a patient with DCM: report of a case. Surg Today. 2011; 42(2):181-4. DOI: 10.1007/s00595-011-0106-4. View

13.

Umezawa T, Higa K, Serikawa M, Yamamoto M, Matsunaga S, Shimazaki J . Proliferative activity of skeletal myoblast sheet by paracrine effects of mesenchymal stem cells. J Oral Biosci. 2020; 58(4):158-166. DOI: 10.1016/j.job.2016.05.005. View

14.

Ohki T, Yamato M, Ota M, Takagi R, Murakami D, Kondo M . Prevention of esophageal stricture after endoscopic submucosal dissection using tissue-engineered cell sheets. Gastroenterology. 2012; 143(3):582-588.e2. DOI: 10.1053/j.gastro.2012.04.050. View

15.

. Committee on Hearing and Equilibrium guidelines for the evaluation of results of treatment of conductive hearing loss. AmericanAcademy of Otolaryngology-Head and Neck Surgery Ffoundation, Inc. Otolaryngol Head Neck Surg. 1995; 113(3):186-7. DOI: 10.1016/S0194-5998(95)70103-6. View

16.

Kanzaki M, Takagi R, Washio K, Kokubo M, Mitsuboshi S, Isaka T . Bio-artificial pleura using autologous dermal fibroblast sheets to mitigate air leaks during thoracoscopic lung resection. NPJ Regen Med. 2021; 6(1):2. PMC: 7782673. DOI: 10.1038/s41536-020-00113-z. View

17.

Yamamoto K, Hama T, Yamato M, Uchimizu H, Sugiyama H, Takagi R . The effect of transplantation of nasal mucosal epithelial cell sheets after middle ear surgery in a rabbit model. Biomaterials. 2014; 42:87-93. DOI: 10.1016/j.biomaterials.2014.11.037. View

18.

Ng M, LINTHICUM Jr F . Long-term effects of Silastic sheeting in the middle ear. Laryngoscope. 1992; 102(10):1097-102. DOI: 10.1288/00005537-199210000-00002. View

19.

Sato M, Yamato M, Mitani G, Takagaki T, Hamahashi K, Nakamura Y . Combined surgery and chondrocyte cell-sheet transplantation improves clinical and structural outcomes in knee osteoarthritis. NPJ Regen Med. 2019; 4:4. PMC: 6384900. DOI: 10.1038/s41536-019-0069-4. View

20.

Yamamoto K, Morino T, Kasai Y, Komori M, Yamato M, Kojima H . Preclinical assessment of transplantable human nasal mucosal epithelial cell sheets. Regen Ther. 2021; 18:59-65. PMC: 8038937. DOI: 10.1016/j.reth.2021.03.006. View