Small RNA Profiling in Provides Insights Into Stress Adaptability

Overview

Journal Front Microbiol

Specialty Microbiology

Date 2021 Nov 22

PMID 34803973

Citations 2

Authors

Yingyu Chen

Wenjun Zhai

Kailun Zhang

Han Liu

Tingting Zhu

Li Su

Luiz Bermudez

Huanchun Chen

Aizhen Guo

Affiliations

Soon will be listed here.

Abstract

encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used and Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.

Citing Articles

complex molecular networks and their regulation: Implications of strain heterogeneity on epigenetic diversity and transcriptome regulation.

Mvubu N, Jacoby K Heliyon. 2023; 9(12):e22611.

PMID: 38046135 PMC: 10686871. DOI: 10.1016/j.heliyon.2023.e22611.

Fatty Acyl-CoA Synthetase D33 Promotes Survival in Hostile Extracellular and Intracellular Microenvironments in the Host.

Zhu Y, Shi H, Tang T, Li Q, Peng Y, Bermudez L Cells. 2023; 12(22).

PMID: 37998345 PMC: 10670722. DOI: 10.3390/cells12222610.

References

Arnvig K, Young D . Identification of small RNAs in Mycobacterium tuberculosis. Mol Microbiol. 2009; 73(3):397-408. PMC: 2764107. DOI: 10.1111/j.1365-2958.2009.06777.x. View

Rastogi S, Singh A, Pant G, Mitra K, Sashidhara K, Krishnan M . Down-regulation of PE11, a cell wall associated esterase, enhances the biofilm growth of Mycobacterium tuberculosis and reduces cell wall virulence lipid levels. Microbiology (Reading). 2017; 163(1):52-61. DOI: 10.1099/mic.0.000417. View

Ramsugit S, Pillay M . Mycobacterium tuberculosis Pili promote adhesion to and invasion of THP-1 macrophages. Jpn J Infect Dis. 2014; 67(6):476-8. DOI: 10.7883/yoken.67.476. View

Wang C, Zhang Q, Tang X, An Y, Li S, Xu H . Effects of CwlM on autolysis and biofilm formation in Mycobacterium tuberculosis and Mycobacterium smegmatis. Int J Med Microbiol. 2018; 309(1):73-83. DOI: 10.1016/j.ijmm.2018.12.002. View

Bansal R, Anil Kumar V, Sevalkar R, Singh P, Sarkar D . Mycobacterium tuberculosis virulence-regulator PhoP interacts with alternative sigma factor SigE during acid-stress response. Mol Microbiol. 2017; 104(3):400-411. DOI: 10.1111/mmi.13635. View

Kwon M, Strobel S . Chemical basis of glycine riboswitch cooperativity. RNA. 2007; 14(1):25-34. PMC: 2151043. DOI: 10.1261/rna.771608. View

Gerrick E, Barbier T, Chase M, Xu R, Francois J, Lin V . Small RNA profiling in identifies MrsI as necessary for an anticipatory iron sparing response. Proc Natl Acad Sci U S A. 2018; 115(25):6464-6469. PMC: 6016810. DOI: 10.1073/pnas.1718003115. View

Gago G, Diacovich L, Gramajo H . Lipid metabolism and its implication in mycobacteria-host interaction. Curr Opin Microbiol. 2017; 41:36-42. PMC: 5862736. DOI: 10.1016/j.mib.2017.11.020. View

Ates L, Ummels R, Commandeur S, van de Weerd R, van der Weerd R, Sparrius M . Essential Role of the ESX-5 Secretion System in Outer Membrane Permeability of Pathogenic Mycobacteria. PLoS Genet. 2015; 11(5):e1005190. PMC: 4418733. DOI: 10.1371/journal.pgen.1005190. View

10.

Rameshwaram N, Singh P, Ghosh S, Mukhopadhyay S . Lipid metabolism and intracellular bacterial virulence: key to next-generation therapeutics. Future Microbiol. 2018; 13:1301-1328. DOI: 10.2217/fmb-2018-0013. View

11.

Mandal M, Lee M, Barrick J, Weinberg Z, Emilsson G, Ruzzo W . A glycine-dependent riboswitch that uses cooperative binding to control gene expression. Science. 2004; 306(5694):275-9. DOI: 10.1126/science.1100829. View

12.

Ritchey L, Tack D, Yakhnin H, Jolley E, Assmann S, Bevilacqua P . Structure-seq2 probing of RNA structure upon amino acid starvation reveals both known and novel RNA switches in . RNA. 2020; 26(10):1431-1447. PMC: 7491331. DOI: 10.1261/rna.075986.120. View

13.

Bychenko O, Skvortsova Y, Ziganshin R, Grigorov A, Aseev L, Ostrik A . Small RNA MTS1338 Confers Pathogenic Properties to Non-Pathogenic . Microorganisms. 2021; 9(2). PMC: 7921967. DOI: 10.3390/microorganisms9020414. View

14.

Mai J, Rao C, Watt J, Sun X, Lin C, Zhang L . Mycobacterium tuberculosis 6C sRNA binds multiple mRNA targets via C-rich loops independent of RNA chaperones. Nucleic Acids Res. 2019; 47(8):4292-4307. PMC: 6486639. DOI: 10.1093/nar/gkz149. View

15.

Zhang Y, Li J, Li B, Wang J, Liu C . Mycobacterium tuberculosis Mce3C promotes mycobacteria entry into macrophages through activation of β2 integrin-mediated signalling pathway. Cell Microbiol. 2017; 20(2). DOI: 10.1111/cmi.12800. View

16.

Xiang X, Deng W, Liu M, Xie J . Mycobacterium biofilms: factors involved in development, dispersal, and therapeutic strategies against biofilm-relevant pathogens. Crit Rev Eukaryot Gene Expr. 2014; 24(3):269-79. DOI: 10.1615/critreveukaryotgeneexpr.2014010545. View

17.

Gong C, Klumpp S . Modeling sRNA-Regulated Plasmid Maintenance. PLoS One. 2017; 12(1):e0169703. PMC: 5234814. DOI: 10.1371/journal.pone.0169703. View

18.

Sun J, Champion P, Bigi F . Editorial: Cellular and Molecular Mechanisms of Virulence. Front Cell Infect Microbiol. 2019; 9:331. PMC: 6794420. DOI: 10.3389/fcimb.2019.00331. View

19.

Alonso-Hearn M, Eckstein T, Sommer S, Bermudez L . A Mycobacterium avium subsp. paratuberculosis LuxR regulates cell envelope and virulence. Innate Immun. 2009; 16(4):235-47. DOI: 10.1177/1753425909339811. View

20.

Westermann A . Regulatory RNAs in Virulence and Host-Microbe Interactions. Microbiol Spectr. 2018; 6(4). PMC: 11633609. DOI: 10.1128/microbiolspec.RWR-0002-2017. View