Cytotoxic Potentials of Silibinin Assisted Silver Nanoparticles on Human Colorectal HT-29 Cancer Cells

Overview

Journal Bioinformation

Specialty Biology

Date 2021 Nov 22

PMID 34803254

Citations 1

Authors

Kiren Jackson

Ezhilarasan Devaraj

Thangavelu Lakshmi

Shanmugam Rajeshkumar

Kamal Dua

Dinesh Kumar Chellappan

Subramanian Raghunandhakumar

Affiliations

Soon will be listed here.

Abstract

It is of interest to study the cytotoxicity of silibinin assisted silver nanoparticles in human colorectal (HT-29) cancer cells. Silver nanoparticles were synthesized using silibinin as a reducing agent. The synthesized silibinin assisted silver nanoparticles ( SSNPs) were characterized and analyzed using a transmission electron microscope and spectrophotometer. The SSNPs synthesized in this study are spherical and their size ranges from 10 to 80 nm. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/mL) of SSNPs and cytotoxicity was evaluated. The apoptosis was using flow cytometry. p53 protein expression using western blot. SSNPs are induced a decrease in viability and increased concentration-dependent cytotoxicity in HT-29 cells. SSNPs treatment also caused apoptosis-related morphological changes. SSNPs treatments at 8 and 16 ng/ml showed a prominent apoptotic change i.e., 70.3% and 83.6% respectively, and decreased viability of HT-29 cells 20% and 11.2% respectively as compared to control cells. SSNPs treatments induced p53 expression in HT-29 cells. Data shows that SSNPs have the potential to induce apoptosis in colorectal cancer cells. This provides insights for the further evaluation of SSNPs in fighting colon cancer.

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References

Idrees M, Tejani M . Current Treatment Strategies for Elderly Patients with Metastatic Colon Cancer. Cureus. 2019; 11(5):e4713. PMC: 6650188. DOI: 10.7759/cureus.4713. View

Brody H . Colorectal cancer. Nature. 2015; 521(7551):S1. DOI: 10.1038/521S1a. View

Ezhilarasan D, Evraerts J, Brice S, Buc-Calderon P, Karthikeyan S, Sokal E . Silibinin Inhibits Proliferation and Migration of Human Hepatic Stellate LX-2 Cells. J Clin Exp Hepatol. 2016; 6(3):167-174. PMC: 5052367. DOI: 10.1016/j.jceh.2016.01.002. View

Murugesan K, Koroth J, Srinivasan P, Singh A, Mukundan S, Karki S . Effects of green synthesised silver nanoparticles (ST06-AgNPs) using curcumin derivative (ST06) on human cervical cancer cells (HeLa) in vitro and EAC tumor bearing mice models. Int J Nanomedicine. 2019; 14:5257-5270. PMC: 6646051. DOI: 10.2147/IJN.S202404. View

Azeem S, Gillani S, Siddiqui A, Jandrajupalli S, Poh V, Syed Sulaiman S . Diet and Colorectal Cancer Risk in Asia--a Systematic Review. Asian Pac J Cancer Prev. 2015; 16(13):5389-96. DOI: 10.7314/apjcp.2015.16.13.5389. View

Rohit Singh T, Ezhilarasan D . Ethanolic Extract of (L.) Pers., Induces Apoptosis and Cell Cycle Arrest in HepG2 Cells. Nutr Cancer. 2019; 72(1):146-156. DOI: 10.1080/01635581.2019.1616780. View

Sohaib M , D E . Carbamazepine, a Histone Deacetylase Inhibitor Induces Apoptosis in Human Colon Adenocarcinoma Cell Line HT-29. J Gastrointest Cancer. 2019; 51(2):564-570. DOI: 10.1007/s12029-019-00286-x. View

Raina K, Agarwal R . Combinatorial strategies for cancer eradication by silibinin and cytotoxic agents: efficacy and mechanisms. Acta Pharmacol Sin. 2007; 28(9):1466-75. DOI: 10.1111/j.1745-7254.2007.00691.x. View

Ezhilarasan D, Karthikeyan S . Silibinin alleviates N-nitrosodimethylamine-induced glutathione dysregulation and hepatotoxicity in rats. Chin J Nat Med. 2016; 14(1):40-7. DOI: 10.3724/SP.J.1009.2016.00040. View

10.

Kuipers E, Grady W, Lieberman D, Seufferlein T, Sung J, Boelens P . Colorectal cancer. Nat Rev Dis Primers. 2016; 1:15065. PMC: 4874655. DOI: 10.1038/nrdp.2015.65. View

11.

Taatjes D, Roth J . In focus in HCB. Histochem Cell Biol. 2020; 153(3):129-133. DOI: 10.1007/s00418-020-01852-2. View

12.

Takke A, Shende P . Nanotherapeutic silibinin: An insight of phytomedicine in healthcare reformation. Nanomedicine. 2019; 21:102057. DOI: 10.1016/j.nano.2019.102057. View

13.

Akhtar R, Ali M, Mahmood S, Sanyal S . Anti-proliferative action of silibinin on human colon adenomatous cancer HT-29 cells. Nutr Hosp. 2014; 29(2):388-92. DOI: 10.3305/nh.2014.29.2.7080. View

14.

Hafez D, Elkhodairy K, Teleb M, Elzoghby A . Nanomedicine-based approaches for improved delivery of phyto-therapeutics for cancer therapy. Expert Opin Drug Deliv. 2020; 17(3):279-285. DOI: 10.1080/17425247.2020.1723542. View

15.

Raj R K, D E, S R . β-Sitosterol-assisted silver nanoparticles activates Nrf2 and triggers mitochondrial apoptosis via oxidative stress in human hepatocellular cancer cell line. J Biomed Mater Res A. 2020; 108(9):1899-1908. DOI: 10.1002/jbm.a.36953. View

16.

Khan S, Zaidi S, Alouffi A, Hassan I, Imran A, Khan R . Computational Proteome-Wide Study for the Prediction of Protein Targeting in Host Cell Organelles and Their Implication in Development of Colon Cancer. ACS Omega. 2020; 5(13):7254-7261. PMC: 7143404. DOI: 10.1021/acsomega.9b04042. View

17.

Agarwal C, Singh R, Dhanalakshmi S, Tyagi A, Tecklenburg M, Sclafani R . Silibinin upregulates the expression of cyclin-dependent kinase inhibitors and causes cell cycle arrest and apoptosis in human colon carcinoma HT-29 cells. Oncogene. 2003; 22(51):8271-82. DOI: 10.1038/sj.onc.1207158. View

18.

Huang Y, Lu X, Lu X . Cytotoxic Mechanism for Silver Nanoparticles Based High-Content Cellomics and Transcriptome Sequencing. J Biomed Nanotechnol. 2019; 15(7):1401-1414. DOI: 10.1166/jbn.2019.2785. View

19.

Ahmadian E, Dizaj S, Rahimpour E, Hasanzadeh A, Eftekhari A, Hosain Zadegan H . Effect of silver nanoparticles in the induction of apoptosis on human hepatocellular carcinoma (HepG2) cell line. Mater Sci Eng C Mater Biol Appl. 2018; 93:465-471. DOI: 10.1016/j.msec.2018.08.027. View

20.

Huy T, Huyen P, Le A, Tonezzer M . Recent Advances of Silver Nanoparticles in Cancer Diagnosis and Treatment. Anticancer Agents Med Chem. 2019; 20(11):1276-1287. DOI: 10.2174/1871520619666190710121727. View