Detection of Antibodies to Human Immunodeficiency Virus (HIV) in Eluates from Whole Blood Impregnated Filter Paper Discs

Overview

Journal J Virol Methods

Specialty Microbiology

Date 1987 Oct 1

PMID 3480292

Citations 11

Authors

B O Lindhardt

I C Bygbjerg

K Ulrich

H D Petersen

I Lausen

B Frederiksen

Affiliations

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Abstract

A method for elution of HIV antibodies from whole blood or serum impregnated filter paper discs was developed. The results from testing of 73 eluates in an enzyme linked immunosorbent assay and the immunoblotting test agreed with the results obtained by ordinary serum testing. Significant loss of antibody activity was not observed, neither in the eluates after storage for 1 mth at -20 degrees C nor in the filter paper discs after storage for 3 mths at +4 degrees C. This technique may be useful in facilitating sample collection and transportation, particularly in remote areas of the world.

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