Conservation of Griseofulvin Genes in the Gsf Gene Cluster Among Fungal Genomes

Overview

Journal G3 (Bethesda)

Publisher Oxford University Press

Specialties Genetics
Molecular Biology

Date 2021 Nov 18

PMID 34792561

Citations 4

Authors

Parisa Aris

Lihong Yan

Yulong Wei

Ying Chang

Bihong Shi

Xuhua Xia

Affiliations

Soon will be listed here.

Abstract

The polyketide griseofulvin is a natural antifungal compound and research in griseofulvin has been key in establishing our current understanding of polyketide biosynthesis. Nevertheless, the griseofulvin gsf biosynthetic gene cluster (BGC) remains poorly understood in most fungal species, including Penicillium griseofulvum where griseofulvin was first isolated. To elucidate essential genes involved in griseofulvin biosynthesis, we performed third-generation sequencing to obtain the genome of P. griseofulvum strain D-756. Furthermore, we gathered publicly available genome of 11 other fungal species in which gsf gene cluster was identified. In a comparative genome analysis, we annotated and compared the gsf BGC of all 12 fungal genomes. Our findings show no gene rearrangements at the gsf BGC. Furthermore, seven gsf genes are conserved by most genomes surveyed whereas the remaining six were poorly conserved. This study provides new insights into differences between gsf BGC and suggests that seven gsf genes are essential in griseofulvin production.

Citing Articles

Computational Design of Novel Griseofulvin Derivatives Demonstrating Potential Antibacterial Activity: Insights from Molecular Docking and Molecular Dynamics Simulation.

Aris P, Mohamadzadeh M, Zarei M, Xia X Int J Mol Sci. 2024; 25(2).

PMID: 38256112 PMC: 10816260. DOI: 10.3390/ijms25021039.

In Silico Exploration of Microtubule Agent Griseofulvin and Its Derivatives Interactions with Different Human β-Tubulin Isotypes.

Aris P, Mohamadzadeh M, Kruglikov A, Askari Rad M, Xia X Molecules. 2023; 28(5).

PMID: 36903629 PMC: 10005519. DOI: 10.3390/molecules28052384.

Griseofulvin: An Updated Overview of Old and Current Knowledge.

Aris P, Wei Y, Mohamadzadeh M, Xia X Molecules. 2022; 27(20).

PMID: 36296627 PMC: 9610072. DOI: 10.3390/molecules27207034.

In Silico Molecular Dynamics of Griseofulvin and Its Derivatives Revealed Potential Therapeutic Applications for COVID-19.

Aris P, Mohamadzadeh M, Wei Y, Xia X Int J Mol Sci. 2022; 23(13).

PMID: 35805893 PMC: 9267096. DOI: 10.3390/ijms23136889.

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