Candida Species Biotypes and Polyclonality of Potentially Virulent Candida Albicans Isolated from Oral Cavity of Patients with Orofacial Clefts

Overview

Journal Clin Oral Investig

Specialty Dentistry

Date 2021 Nov 18

PMID 34791549

Citations 3

Authors

Marcelo Fabiano Gomes Boriollo

Mateus Cardoso Oliveira

Vanessa Bassinello

Paula Cristina Anibal

Thaisla Andrielle da Silva

Jeferson Junior da Silva

Rodrigo Carlos Bassi

Manoel Francisco Rodrigues Netto

Carlos Tadeu Dos Santos Dias

Jose Francisco Hofling

Affiliations

Soon will be listed here.

Abstract

Objectives: This study evaluated the incidence of Candida species, and the genetic diversity and virulence of C. albicans of the oral cavity from patients with cleft lip and palate (CLP).

Materials And Methods: Oral samples were investigated by microbiological and species-specific PCR methods. The genetic diversity of C. albicans was established using isoenzyme markers, Nei's statistics, and clustering analysis. Hydrolytic enzymes (SAPs and PLs) were analyzed in vitro.

Results: Oral colonization by Candida species was observed in 29 patients with CLP (65.9%), and C. albicans was highly prevalent. SAP and PL activities were observed in 100% and 51.9% of isolates, respectively. High genetic diversity and patterns of monoclonal and polyclonal oral colonization by C. albicans were observed among patients with CLP. Two major polymorphic taxa (A and B) and other minor polymorphic taxa (C to J) were identified. Only one of the 16 clusters (taxon A) harbored strains from patients with and without CLP, whereas other clusters harbored strains exclusively from CLP patients.

Conclusions: The anatomical conditions of the oral cavity of patients with CLP contribute to the high incidence of Candida species (C. albicans, C. krusei, C. tropicalis, and/or Candida spp.). Data suggest high genetic diversity of potentially virulent C. albicans strains in the oral cavity of CLP patients.

Clinical Relevance: Microbiological niches in orofacial clefts can contribute to the emergence of a relative clinical genotypic identity of C. albicans. However, orofacial rehabilitation centers can contribute to the direct and indirect sources of transmission and propagation of Candida species.

Citing Articles

Oral health in patients with cleft lip and palate: a systematic literature review and meta-analysis of periodontal and dental disease and oral microbiota (part 1).

Pardo A, Vanti V, Lonardi F, Signoriello A, Lobbia G, Lombardo G BMC Oral Health. 2025; 25(1):154.

PMID: 39881261 PMC: 11776186. DOI: 10.1186/s12903-025-05494-5.

Oral Species and MRSA Strains in Patients with Orofacial Clefts Undergoing Surgical Rehabilitation Diagnosed by MALDI-TOF MS.

Oliveira M, Boriollo M, Souza A, Silva T, Silva J, Magalhaes-Guedes K Pathogens. 2024; 13(9).

PMID: 39338954 PMC: 11434827. DOI: 10.3390/pathogens13090763.

Incidence and prevalence of oral candidal colonization in patients with cleft lip and palate.

Khan I, Ahmedi S, Ahmad T, Rizvi M, Manzoor N Natl J Maxillofac Surg. 2023; 14(1):72-78.

PMID: 37273448 PMC: 10235736. DOI: 10.4103/njms.njms_127_22.

Characterizing the microbiota of cleft lip and palate patients: a comprehensive review.

Gershater E, Liu Y, Xue B, Shin M, Koo H, Zheng Z Front Cell Infect Microbiol. 2023; 13:1159455.

PMID: 37143743 PMC: 10152472. DOI: 10.3389/fcimb.2023.1159455.

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