Effect of AMY1 Copy Number Variation and Various Doses of Starch Intake on Glucose Homeostasis: Data from a Cross-sectional Observational Study and a Crossover Meal Study

Overview

Journal Genes Nutr

Publisher Biomed Central

Date 2021 Nov 18

PMID 34789141

Citations 3

Authors

Mary Farrell

Stina Ramne

Phebee Gouinguenet

Louise Brunkwall

Ulrika Ericson

Anne Raben

Peter M Nilsson

Marju Orho-Melander

Yvonne Granfeldt

Juscelino Tovar

Emily Sonestedt

Affiliations

Soon will be listed here.

Abstract

Background: Copy number (CN) variation (CNV) of the salivary amylase gene (AMY1) influences the ability to digest starch and may influence glucose homeostasis, obesity and gut microbiota composition. Hence, the aim was to examine the association of AMY1 CNV with fasting glucose, BMI, and gut microbiota composition considering habitual starch intake and to investigate the effect of AMY1 CNV on the postprandial response after two different starch doses.

Methods: The Malmö Offspring Study (n = 1764, 18-71 years) was used to assess interaction effects between AMY1 CNV (genotyped by digital droplet polymerase chain reaction) and starch intake (assessed by 4-day food records) on fasting glucose, BMI, and 64 gut bacteria (16S rRNA sequencing). Participants with low (≤ 4 copies, n = 9) and high (≥ 10 copies, n = 10) AMY1 CN were recruited for a crossover meal study to compare postprandial glycemic and insulinemic responses to 40 g and 80 g starch from white wheat bread.

Results: In the observational study, no overall associations were found between AMY1 CNV and fasting glucose, BMI, or gut microbiota composition. However, interaction effects between AMY1 CNV and habitual starch intake on fasting glucose (P = 0.03) and BMI (P = 0.05) were observed, suggesting inverse associations between AMY1 CNV and fasting glucose and BMI at high starch intake levels and positive association at low starch intake levels. No associations with the gut microbiota were observed. In the meal study, increased postprandial glucose (P = 0.02) and insulin (P = 0.05) were observed in those with high AMY1 CN after consuming 40 g starch. This difference was smaller and nonsignificant after consuming 80 g starch.

Conclusions: Starch intake modified the observed association between AMY1 CNV and fasting glucose and BMI. Furthermore, depending on the starch dose, a higher postprandial glucose and insulin response was observed in individuals with high AMY1 CN than in those with low AMY1 CN.

Trial Registration: ClinicalTrials.gov , NCT03974126 . Registered 4 June 2019-retrospectively registered.

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References

Usher C, Handsaker R, Esko T, Tuke M, Weedon M, Hastie A . Structural forms of the human amylase locus and their relationships to SNPs, haplotypes and obesity. Nat Genet. 2015; 47(8):921-5. PMC: 4712930. DOI: 10.1038/ng.3340. View

Barber T, Bhatti A, Elder P, Ball S, Calvez R, Ramsden D . AMY1 Gene Copy Number Correlates With Glucose Absorption and Visceral Fat Volume, but Not with Insulin Resistance. J Clin Endocrinol Metab. 2020; 105(10). DOI: 10.1210/clinem/dgaa473. View

Al-Akl N, Thompson R, Arredouani A . High plasma salivary α-amylase, but not high AMY1 copy number, associated with low obesity rate in Qatari adults: cross-sectional study. Sci Rep. 2020; 10(1):17918. PMC: 7578794. DOI: 10.1038/s41598-020-74864-6. View

Mandel A, Breslin P . High endogenous salivary amylase activity is associated with improved glycemic homeostasis following starch ingestion in adults. J Nutr. 2012; 142(5):853-8. PMC: 3327743. DOI: 10.3945/jn.111.156984. View

Bonnefond A, Yengo L, Dechaume A, Canouil M, Castelain M, Roger E . Relationship between salivary/pancreatic amylase and body mass index: a systems biology approach. BMC Med. 2017; 15(1):37. PMC: 5322607. DOI: 10.1186/s12916-017-0784-x. View

Peyrot des Gachons C, Breslin P . Salivary Amylase: Digestion and Metabolic Syndrome. Curr Diab Rep. 2016; 16(10):102. PMC: 6825871. DOI: 10.1007/s11892-016-0794-7. View

Heianza Y, Zhou T, Yuhang C, Huang T, Willett W, Hu F . Starch Digestion-Related Amylase Genetic Variants, Diet, and Changes in Adiposity: Analyses in Prospective Cohort Studies and a Randomized Dietary Intervention. Diabetes. 2020; 69(9):1917-1926. PMC: 7458037. DOI: 10.2337/db19-1257. View

Brunkwall L, Jonsson D, Ericson U, Hellstrand S, Kennback C, Ostling G . The Malmö Offspring Study (MOS): design, methods and first results. Eur J Epidemiol. 2020; 36(1):103-116. PMC: 7847466. DOI: 10.1007/s10654-020-00695-4. View

Atkinson F, Hancock D, Petocz P, Brand-Miller J . The physiologic and phenotypic significance of variation in human amylase gene copy number. Am J Clin Nutr. 2018; 108(4):737-748. DOI: 10.1093/ajcn/nqy164. View

10.

Choi Y, Nam Y, Yun J, Park J, Cho B, Son H . Association between salivary amylase (AMY1) gene copy numbers and insulin resistance in asymptomatic Korean men. Diabet Med. 2015; 32(12):1588-95. DOI: 10.1111/dme.12808. View

11.

Yong R, Mustaffa S, Wasan P, Sheng L, Marshall C, Scherer S . Complex Copy Number Variation of AMY1 does not Associate with Obesity in two East Asian Cohorts. Hum Mutat. 2016; 37(7):669-78. DOI: 10.1002/humu.22996. View

12.

Herrmann E, Young W, Rosendale D, Conrad R, Riedel C, Egert M . Determination of Resistant Starch Assimilating Bacteria in Fecal Samples of Mice by RNA-Based Stable Isotope Probing. Front Microbiol. 2017; 8:1331. PMC: 5522855. DOI: 10.3389/fmicb.2017.01331. View

13.

Venkatapoorna C, Ayine P, Parra E, Koenigs T, Phillips M, Babu J . Association of Salivary Amylase () Gene Copy Number with Obesity in Alabama Elementary School Children. Nutrients. 2019; 11(6). PMC: 6627241. DOI: 10.3390/nu11061379. View

14.

Shwan N, Armour J . No Evidence for Association of BMI with Salivary Amylase Gene Copy Number in the UK 1958 Birth Cohort. Obesity (Silver Spring). 2019; 27(9):1533-1538. DOI: 10.1002/oby.22565. View

15.

Vazquez-Moreno M, Mejia-Benitez A, Sharma T, Peralta-Romero J, Locia-Morales D, Klunder-Klunder M . Association of AMY1A/AMY2A copy numbers and AMY1/AMY2 serum enzymatic activity with obesity in Mexican children. Pediatr Obes. 2020; 15(8):e12641. DOI: 10.1111/ijpo.12641. View

16.

Zhang L, Ouyang Y, Li H, Shen L, Ni Y, Fang Q . Metabolic phenotypes and the gut microbiota in response to dietary resistant starch type 2 in normal-weight subjects: a randomized crossover trial. Sci Rep. 2019; 9(1):4736. PMC: 6426958. DOI: 10.1038/s41598-018-38216-9. View

17.

Ze X, Duncan S, Louis P, Flint H . Ruminococcus bromii is a keystone species for the degradation of resistant starch in the human colon. ISME J. 2012; 6(8):1535-43. PMC: 3400402. DOI: 10.1038/ismej.2012.4. View

18.

Viljakainen H, Andersson-Assarsson J, Armenio M, Pekkinen M, Pettersson M, Valta H . Low Copy Number of the AMY1 Locus Is Associated with Early-Onset Female Obesity in Finland. PLoS One. 2015; 10(7):e0131883. PMC: 4489572. DOI: 10.1371/journal.pone.0131883. View

19.

Perry G, Dominy N, Claw K, Lee A, Fiegler H, Redon R . Diet and the evolution of human amylase gene copy number variation. Nat Genet. 2007; 39(10):1256-60. PMC: 2377015. DOI: 10.1038/ng2123. View

20.

Selvaraju V, Venkatapoorna C, Babu J, Geetha T . Salivary Amylase Gene Copy Number Is Associated with the Obesity and Inflammatory Markers in Children. Diabetes Metab Syndr Obes. 2020; 13:1695-1701. PMC: 7245430. DOI: 10.2147/DMSO.S251359. View