Expression Characteristic of and in Acute Myeloid Leukemia

() and () expression characteristics in acute myeloid leukemia (AML) remain unknown. This study investigated mRNA and membrane protein expression of two isoforms in AML cell lines and de novo patients by using RT-PCR and flow cytometry, and analyzed the promoter methylation state by utilizing RQ-MSP. was the dominant isoform. mRNA expression rate and abundance were elevated in AML in comparison with controls ( = 0.000 and 0.000), while no significant difference in ( = 0.802, = 0.398). Membrane protein levels of isoforms in AML was higher than controls, detected by total expression rate ( = 0.002), total mean fluorescence intensity (MFI) ratio of blast cells and lymphocytes (MFI ratio) ( = 0.000), and MFI ratio ( = 0.005). Compared with group, patients had older age, lower mutation, higher mutation, and declining complete remission (CR) rates ( = 0.026, 0.012, 0.047, and 0.028). In group, there was a trend toward older age, M4 and M5, worse karyotype, and lower CR rates, although with no marked difference ( > 0.05). The overall survival (OS) of and groups were shorter than that of and groups in the whole and non-M3 AML cohorts ( = 0.006 and 0.046; = 0.003 and 0.032). Besides, an unmethylated promoter was identified. In conclusion, mRNA and total membrane protein tended to have potential diagnostic value for AML. Specifically, high mRNA and total membrane protein expression indicate worse OS. and expression are methylation-independent.