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Digital PCR to Quantify ChAdOx1 NCoV-19 Copies in Blood and Tissues

Overview
Journal Mol Ther Methods Clin Dev
Publisher Cell Press
Date 2021 Nov 17
PMID 34786434
Citations 4
Authors
Anita Badbaran
Reiner K Mailer
Christine Dahlke
Jannis Woens
Anahita Fathi
Sibylle C Mellinghoff
Thomas Renne
Marylyn M Addo
Kristoffer Riecken
Boris Fehse
Affiliations
Soon will be listed here.
Abstract

Vaccination with the adenoviral-vector-based AstraZeneca ChAdOx1 nCov-19 (Vaxzevria) vaccine is efficient and safe. However, in rare cases vaccinated individuals developed life-threatening thrombotic complications, including thrombosis in cerebral sinus and splanchnic veins. Monitoring of the applied vector represents an important precondition to study the molecular mechanisms underlying vaccine-driven adverse effects now referred to as vaccine-induced immune thrombotic thrombocytopenia (VITT). We previously have shown that digital PCR (dPCR) is an excellent tool to quantify transgene copies . Here, we present a highly sensitive dPCR for quantification of ChAdOx1 nCoV-19 copies. Using this method, we quantified vector copies in human plasma 24, 72, and 168 h post vaccination and in a variety of murine tissues in an experimental vaccination model 30 min post injection. We describe a method for high-sensitivity quantitative detection of ChAdOx1 nCoV-19 with possible implications to elucidate the mechanisms of severe ChAdOx1 nCov-19 vaccine complications.

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Revealing the Mysteries of Acute Myeloid Leukemia: From Quantitative PCR through Next-Generation Sequencing and Systemic Metabolomic Profiling.

Panuzzo C, Jovanovski A, Ali M, Cilloni D, Pergolizzi B J Clin Med. 2022; 11(3).

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Thrombocytopenia in COVID‑19 and vaccine‑induced thrombotic thrombocytopenia.

Geronikolou S, Takan I, Pavlopoulou A, Mantzourani M, Chrousos G Int J Mol Med. 2022; 49(3).

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Untangling the Intricacies of Infection, Thrombosis, Vaccination, and Antiphospholipid Antibodies for COVID-19.

Cimolai N SN Compr Clin Med. 2021; 3(10):2093-2108.

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