LncRNA TMPO-AS1 Serves As a Sponge for MiR-4731-5p Modulating Breast Cancer Progression Through FOXM1

Overview

Journal Am J Transl Res

Specialty General Medicine

Date 2021 Nov 17

PMID 34786045

Citations 4

Authors

Ying Wang

Jun Ma

Ruiqing Li

Xiaokang Gao

Hongli Wang

Guoqin Jiang

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the function of IncRNA-TMPO-AS1 in breast cancer (BC) and to further explore its molecular mechanism.

Methods: TMPO-AS1, miR-4731-5p and FOXM1 were quantitatively determined using qRT-PCR. CCK-8 assays, plate cloning experiments, wound healing and Transwell assays, and flow cytometry were used to assess the biological behaviors of BC cells. Dual-luciferase reporter assays were used to assess the interactions between TMPO-AS1 and its downstream targets. The apoptosis and cell cycle-related proteins were quantitatively determined using Western blot.

Results: In the BC tissues and cells, TMPO-AS1 was significantly increased (P<0.05). Functional studies suggest that the knockdown of TMPO-AS1 tremendously restrains tumor cell growth and migration (P<0.05). Mechanically, TMPO-AS1 negatively regulates miR-4731-5p and influences the progression of BC through the miR-4731-5p/FOXM1 axis.

Conclusion: LncRNA TMPO-AS1spongess miR-4731-5p to modulate BC progression through FOXM1.

Citing Articles

Soft Tissue Ewing Sarcoma Cell Drug Resistance Revisited: A Systems Biology Approach.

Asfa S, Toy H, Arshinchi Bonab R, Chrousos G, Pavlopoulou A, Geronikolou S Int J Environ Res Public Health. 2023; 20(13).

PMID: 37444135 PMC: 10341845. DOI: 10.3390/ijerph20136288.

A long non-coding RNA with important roles in the carcinogenesis.

Ghafouri-Fard S, Askari A, Hussen B, Taheri M, Mokhtari M Front Cell Dev Biol. 2022; 10:1037149.

PMID: 36467407 PMC: 9709449. DOI: 10.3389/fcell.2022.1037149.

Docetaxel-resistant triple-negative breast cancer cell-derived exosomal lncRNA LINC00667 reduces the chemosensitivity of breast cancer cells to docetaxel <em>via</em> targeting miR-200b-3p/Bcl-2 axis.

Li J, Kang J, Liu W, Liu J, Pan G, Mao A Eur J Histochem. 2022; 66(4).

PMID: 36281649 PMC: 9667572. DOI: 10.4081/ejh.2022.3529.

miR-4731-5p Enhances Apoptosis and Alleviates Epithelial-Mesenchymal Transition through Targeting RPLP0 in Non-Small-Cell Lung Cancer.

Chang C, Xu M J Oncol. 2022; 2022:3793318.

PMID: 35342398 PMC: 8947863. DOI: 10.1155/2022/3793318.

References

Laoukili J, Stahl M, Medema R . FoxM1: at the crossroads of ageing and cancer. Biochim Biophys Acta. 2006; 1775(1):92-102. DOI: 10.1016/j.bbcan.2006.08.006. View

Mattick J, Rinn J . Discovery and annotation of long noncoding RNAs. Nat Struct Mol Biol. 2015; 22(1):5-7. DOI: 10.1038/nsmb.2942. View

Yamamura S, Imai-Sumida M, Tanaka Y, Dahiya R . Interaction and cross-talk between non-coding RNAs. Cell Mol Life Sci. 2017; 75(3):467-484. PMC: 5765200. DOI: 10.1007/s00018-017-2626-6. View

Wang B, Wang H, Yang Z . MiR-122 inhibits cell proliferation and tumorigenesis of breast cancer by targeting IGF1R. PLoS One. 2012; 7(10):e47053. PMC: 3466252. DOI: 10.1371/journal.pone.0047053. View

Autier P, Hery C, Haukka J, Boniol M, Byrnes G . Advanced breast cancer and breast cancer mortality in randomized controlled trials on mammography screening. J Clin Oncol. 2009; 27(35):5919-23. DOI: 10.1200/JCO.2009.22.7041. View

Liu Y, Yang Y, Wang T, Wang L, Wang X, Li T . Long non-coding RNA CCAL promotes hepatocellular carcinoma progression by regulating AP-2α and Wnt/β-catenin pathway. Int J Biol Macromol. 2017; 109:424-434. DOI: 10.1016/j.ijbiomac.2017.12.110. View

Yin Y, Yan P, Lu J, Song G, Zhu Y, Li Z . Opposing Roles for the lncRNA Haunt and Its Genomic Locus in Regulating HOXA Gene Activation during Embryonic Stem Cell Differentiation. Cell Stem Cell. 2015; 16(5):504-16. DOI: 10.1016/j.stem.2015.03.007. View

Cui H, Zhao J . LncRNA TMPO-AS1 serves as a ceRNA to promote osteosarcoma tumorigenesis by regulating miR-199a-5p/WNT7B axis. J Cell Biochem. 2019; 121(3):2284-2293. DOI: 10.1002/jcb.29451. View

Liz J, Esteller M . lncRNAs and microRNAs with a role in cancer development. Biochim Biophys Acta. 2015; 1859(1):169-76. DOI: 10.1016/j.bbagrm.2015.06.015. View

10.

Stark M, Tom L, Boyle G, Bonazzi V, Soyer H, Herington A . The "melanoma-enriched" microRNA miR-4731-5p acts as a tumour suppressor. Oncotarget. 2016; 7(31):49677-49687. PMC: 5226538. DOI: 10.18632/oncotarget.10109. View

11.

Liu M, Dai B, Kang S, Ban K, Huang F, Lang F . FoxM1B is overexpressed in human glioblastomas and critically regulates the tumorigenicity of glioma cells. Cancer Res. 2006; 66(7):3593-602. DOI: 10.1158/0008-5472.CAN-05-2912. View

12.

Huang W, Su X, Yan W, Kong Z, Wang D, Huang Y . Overexpression of AR-regulated lncRNA TMPO-AS1 correlates with tumor progression and poor prognosis in prostate cancer. Prostate. 2018; 78(16):1248-1261. DOI: 10.1002/pros.23700. View

13.

Duan H, Li X, Chen Y, Wang Y, Li Z . LncRNA RHPN1-AS1 promoted cell proliferation, invasion and migration in cervical cancer via the modulation of miR-299-3p/FGF2 axis. Life Sci. 2019; 239:116856. DOI: 10.1016/j.lfs.2019.116856. View

14.

Adams B, Parsons C, Walker L, Zhang W, Slack F . Targeting noncoding RNAs in disease. J Clin Invest. 2017; 127(3):761-771. PMC: 5330746. DOI: 10.1172/JCI84424. View

15.

Yan Z, Zhang W, Xiong Y, Wang Y, Li Z . Long noncoding RNA FLVCR1-AS1 aggravates biological behaviors of glioma cells via targeting miR-4731-5p/E2F2 axis. Biochem Biophys Res Commun. 2019; 521(3):716-720. DOI: 10.1016/j.bbrc.2019.10.106. View

16.

Fang C, Qiu S, Sun F, Li W, Wang Z, Yue B . Long non-coding RNA HNF1A-AS1 mediated repression of miR-34a/SIRT1/p53 feedback loop promotes the metastatic progression of colon cancer by functioning as a competing endogenous RNA. Cancer Lett. 2017; 410:50-62. DOI: 10.1016/j.canlet.2017.09.012. View

17.

Carpenter S, Aiello D, Atianand M, Ricci E, Gandhi P, Hall L . A long noncoding RNA mediates both activation and repression of immune response genes. Science. 2013; 341(6147):789-92. PMC: 4376668. DOI: 10.1126/science.1240925. View

18.

Myatt S, Lam E . The emerging roles of forkhead box (Fox) proteins in cancer. Nat Rev Cancer. 2007; 7(11):847-59. DOI: 10.1038/nrc2223. View

19.

Loayza-Puch F, Agami R . Lncing protein translation to metastasis. EMBO J. 2013; 32(20):2657-8. PMC: 3801441. DOI: 10.1038/emboj.2013.210. View

20.

Kalinichenko V, Major M, Wang X, Petrovic V, Kuechle J, Yoder H . Foxm1b transcription factor is essential for development of hepatocellular carcinomas and is negatively regulated by the p19ARF tumor suppressor. Genes Dev. 2004; 18(7):830-50. PMC: 387422. DOI: 10.1101/gad.1200704. View