The Proline-rich Domain of MML-1 is Biologically Important but Not Required for Localization to Target Promoters

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2021 Nov 15

PMID 34778725

Authors

Ainhoa Ceballos

Ruben Esse

Alla Grishok

Affiliations

Soon will be listed here.

Abstract

The only representative of the MYC superfamily transcription factors in , MML-1 (Myc and Mondo-like 1), was shown to promote extended lifespan in a variety of models and to regulate some aspects of development. This previous research did not involve molecular characterization of MML-1. Here we use available mutant alleles and other reagents to demonstrate that MML-1 is modified by -GlcNAc, binds to promoters of some genes directly regulated by the DOT-1.1 histone methyltransferase complex, and has a role in promoting neuronal migration. Surprisingly, we found that the deletion allele which was considered a null, produces an internally truncated protein resulting from an in-frame deletion. Localization of this truncated product to MML-1 target promoters was not impaired. The deleted region of MML-1 is proline-rich, and its function is poorly understood in mammalian homologs of MML-1. Based on our work and previously published data we conclude that the internal proline-rich region of MML-1 is dispensable for DNA binding but is biologically important.

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