Identification of Feline Foamy Virus-derived MicroRNAs

Overview

Journal Microbes Environ

Specialty Environmental Health

Date 2021 Nov 15

PMID 34776460

Citations 1

Authors

Shiro Aso

Koichi Kitao

Akira Hashimoto-Gotoh

Shoichi Sakaguchi

Takayuki Miyazawa

Affiliations

Soon will be listed here.

Abstract

MicroRNAs (miRNAs) classified as non-coding RNAs regulate various metabolic systems and viral life cycles. To date, numerous DNA viruses, many of which are members of the herpesvirus family, and a relatively small number of RNA viruses, including retroviruses, have been reported to encode and express miRNAs in infected cells. A few retroviruses have been shown to express miRNAs, and foamy viruses (FVs) were initially predicted by computational analyses to possess miRNA-coding regions. Subsequent studies on simian and bovine FVs confirmed the presence of functional and biologically active miRNA expression cassettes. We herein identified feline FV-derived miRNAs using a small RNA deep sequencing ana-lysis. We confirmed their repressive functions on gene expression by dual-luciferase reporter assays. We found that the seed sequences of the miRNAs identified in the present study were conserved among all previously reported FFV isolates. These results suggest that FFV-derived miRNAs play a pivotal role in FFV infection.

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