CRISPR/Cas9-Mediated Mutagenesis Reduces Tomato Resistance to

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Nov 13

PMID 34768853

Citations 7

Authors

Chunxin Liu

Yiyao Zhang

Yinxiao Tan

Tingting Zhao

Xiangyang Xu

Huanhuan Yang

Jingfu Li

Affiliations

Soon will be listed here.

Abstract

() recently caused epidemics of tomato late blight. Our study aimed to identify the function of the gene in response to tomato late blight. To further investigate the function of in tomato resistance to , we studied the effects of gene knock out. The gene was knocked out by CRISPR-Cas9, and the resulting plants ( gene knockout, ) showed reduced resistance to , accompanied by increases in the number of necrotic cells, lesion sizes, and disease index. Furthermore, after infection, the expression levels of pathogenesis-related () genes in plants were significantly lower than those in wild-type (AC) plants, while the number of necrotic cells and the accumulation of reactive oxygen species (ROS) were higher than those in wild-type plants. Taken together, these results indicate that acts as a positive regulator of tomato resistance to infection by regulating the ROS level and the expression level of genes.

Citing Articles

Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.

Mascarenhas M, Nascimento F, Rocha A, Ferreira M, Oliveira W, Lino L Curr Issues Mol Biol. 2024; 46(10):11086-11123.

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Revolutionizing Tomato Cultivation: CRISPR/Cas9 Mediated Biotic Stress Resistance.

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