Molecular Perturbation Effects in AFM-Based Tip-Enhanced Raman Spectroscopy: Contact Versus Tapping Mode

Overview

Journal Anal Chem

Specialty Chemistry

Date 2021 Nov 12

PMID 34767337

Citations 5

Authors

Giovanni Luca Bartolomeo

Yao Zhang

Naresh Kumar

Renato Zenobi

Affiliations

Soon will be listed here.

Abstract

Tip-enhanced Raman spectroscopy (TERS) is a powerful tool for nondestructive and label-free surface chemical characterization at nanometer length scales. However, despite being considered nondestructive, the interaction of the TERS probe used in the analysis can alter the molecular organization of the sample. In this study, we investigate the role of the atomic force microscopy (AFM) feedback (contact mode and tapping mode) on molecular perturbation in TERS analysis of soft samples using a self-assembled monolayer (SAM) of 2-chloro-4-nitrobenzene-1-thiol (Cl-NBT) as a test sample. Surprisingly, the tapping mode shows a consistently higher TERS signal resulting from a minimal perturbation of the Cl-NBT SAM compared to the contact mode. This study provides novel insights into the choice of the correct AFM-TERS operation mode for nanoscale chemical analysis of soft and delicate samples and is expected to expedite the growing application of TERS in this area.

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