Modulating Effects of the Synergistic Combination of Extracts of Herbal Drugs on Cyclophosphamide-induced Immunosuppressed Mice

Overview

Journal Saudi J Biol Sci

Specialty Biology

Date 2021 Nov 12

PMID 34764748

Citations 7

Authors

Abida Parveen

Sultan Zahiruddin

Nidhi Agarwal

Muhammad Akhtar Siddiqui

Shahid Husain Ansari

Sayeed Ahmad

Affiliations

Soon will be listed here.

Abstract

Objective: Taking leads from the available research, we aimed to develop a synergy-based herbal combination of (TC), (PE) and (PN) Also, evaluating their synergistic effect on CP-induced immunosuppression in mice model and exploring the possible mechanisms involved in reversing the damage.

Methodology: The immunomodulatory activity of combination, of TC stem, PE fruits, and PN dried fruits, was determined by assays (splenocyte proliferation and pinocytic activity of peritoneal macrophages of mice) and study using CP-induced immunosuppression model in Swiss Albino mice. The ratio was optimized for combining three by MTT assay. The combination was further evaluated for anti-oxidant activity by DPPH scavenging method and quantified for its bioactive metabolites by HPTLC. Serum collected on day 0, 4, 7 and 14 was employed for estimation of haematogram (haematocrit, TLC, DLC, and haemoglobin, etc) and immune parameters (IL-10, IL-6 and TNF-α) by ELISA.

Results: The study demonstrated, that combination of herbal extracts at an intermediate dose could inhibit the proliferation of spleen cells and peritoneal macrophages (P ≤ 0.0001) and induce suppression of pro-inflammatory mediators, and also certified that combination exerts synergized effects. The results showed that the combination possess potential antioxidant activity by DPPH scavenging method (IC-113.5 µg/ml). It was identified that combination significantly (P ≤ 0.0001) improved the immune markers, haematogram parameters, and histological parameters, with maximum protection offered by an intermediate dose.

Conclusion: The results suggested that present combination could be further explored clinically as potent synergy-based therapeutic approach for immune modulation.

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References

Kraus M . Splenic histology and histopathology: an update. Semin Diagn Pathol. 2003; 20(2):84-93. DOI: 10.1016/s0740-2570(03)00024-8. View

Sharififar F, Pournourmohammadi S, Arabnejad M . Immunomodulatory activity of aqueous extract of Achillea wilhelmsii C. Koch in mice. Indian J Exp Biol. 2009; 47(8):668-71. View

Cuneo A, Autieri M . Expression and function of anti-inflammatory interleukins: the other side of the vascular response to injury. Curr Vasc Pharmacol. 2009; 7(3):267-76. PMC: 3290808. DOI: 10.2174/157016109788340721. View

Polu P, Nayanbhirama U, Khan S, Maheswari R . Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd). BMC Complement Altern Med. 2017; 17(1):457. PMC: 5594595. DOI: 10.1186/s12906-017-1953-3. View

Wang S, Huang S, Ye Q, Zeng X, Yu H, Qi D . Prevention of Cyclophosphamide-Induced Immunosuppression in Mice with the Antimicrobial Peptide Sublancin. J Immunol Res. 2018; 2018:4353580. PMC: 5964538. DOI: 10.1155/2018/4353580. View

Parveen S, Ansari M, Parveen R, Khan W, Ahmad S, Husain S . Chromatography Based Metabolomics and Screening of Leaf Extract for Its Antidiabetic Potential. Evid Based Complement Alternat Med. 2019; 2019:7523159. PMC: 6339760. DOI: 10.1155/2019/7523159. View

Sakthivel K, Guruvayoorappan C . Acacia ferruginea inhibits cyclophosphamide-induced immunosuppression and urotoxicity by modulating cytokines in mice. J Immunotoxicol. 2014; 12(2):154-63. DOI: 10.3109/1547691X.2014.914988. View

Salem M, Al-Khami A, El-Nagaar S, Zidan A, Al-Sharkawi I, Diaz-Montero C . Kinetics of rebounding of lymphoid and myeloid cells in mouse peripheral blood, spleen and bone marrow after treatment with cyclophosphamide. Cell Immunol. 2012; 276(1-2):67-74. PMC: 3787597. DOI: 10.1016/j.cellimm.2012.03.010. View

Shi L, Fu Y . Isolation, purification, and immunomodulatory activity in vitro of three polysaccharides from roots of Cudrania tricuspidata. Acta Biochim Biophys Sin (Shanghai). 2011; 43(5):418-24. DOI: 10.1093/abbs/gmr024. View

10.

Jantan I, Haque M, Ilangkovan M, Arshad L . An Insight Into the Modulatory Effects and Mechanisms of Action of Species and Their Bioactive Metabolites on the Immune System. Front Pharmacol. 2019; 10:878. PMC: 6693410. DOI: 10.3389/fphar.2019.00878. View

11.

Gorgani L, Mohammadi M, Najafpour G, Nikzad M . Piperine-The Bioactive Compound of Black Pepper: From Isolation to Medicinal Formulations. Compr Rev Food Sci Food Saf. 2020; 16(1):124-140. DOI: 10.1111/1541-4337.12246. View

12.

El-Naggar S, Alm-Eldeen A, Germoush M, El-Boray K, Elgebaly H . Ameliorative effect of propolis against cyclophosphamide-induced toxicity in mice. Pharm Biol. 2014; 53(2):235-41. DOI: 10.3109/13880209.2014.914230. View

13.

Chen X, Li J, Wang H, Cheng W, Zhang L, Ge J . Immunomodulating effects of fractioned polysaccharides isolated from Yu-Ping-Feng-Powder in cyclophosphamide-treated mice. Am J Chin Med. 2006; 34(4):631-41. DOI: 10.1142/S0192415X06004168. View

14.

Pass G, Carrie D, Boylan M, Lorimore S, Wright E, Houston B . Role of hepatic cytochrome p450s in the pharmacokinetics and toxicity of cyclophosphamide: studies with the hepatic cytochrome p450 reductase null mouse. Cancer Res. 2005; 65(10):4211-7. DOI: 10.1158/0008-5472.CAN-04-4103. View

15.

BERENBAUM M . Synergy, additivism and antagonism in immunosuppression. A critical review. Clin Exp Immunol. 1977; 28(1):1-18. PMC: 1540873. View

16.

Dirchwolf M, Podhorzer A, Marino M, Shulman C, Cartier M, Zunino M . Immune dysfunction in cirrhosis: Distinct cytokines phenotypes according to cirrhosis severity. Cytokine. 2015; 77:14-25. DOI: 10.1016/j.cyto.2015.10.006. View

17.

Shruthi S, Vijayalaxmi K . Antigenotoxic effects of a polyherbal drug septilin against the genotoxicity of cyclophosphamide in mice. Toxicol Rep. 2017; 3:563-571. PMC: 5615931. DOI: 10.1016/j.toxrep.2016.07.001. View

18.

Tungmunnithum D, Thongboonyou A, Pholboon A, Yangsabai A . Flavonoids and Other Phenolic Compounds from Medicinal Plants for Pharmaceutical and Medical Aspects: An Overview. Medicines (Basel). 2018; 5(3). PMC: 6165118. DOI: 10.3390/medicines5030093. View

19.

Sharma A, Sharma M, Kumar M . Modulatory role of Emblica officinalis fruit extract against arsenic induced oxidative stress in Swiss albino mice. Chem Biol Interact. 2009; 180(1):20-30. DOI: 10.1016/j.cbi.2009.01.012. View

20.

Madaan A, Kanjilal S, Gupta A, Sastry J, Verma R, Singh A . Evaluation of immunostimulatory activity of Chyawanprash using in vitro assays. Indian J Exp Biol. 2015; 53(3):158-63. View