Characterization of Backbone Dynamics Using Solution NMR Spectroscopy to Discern the Functional Plasticity of Structurally Analogous Proteins

Overview

Journal STAR Protoc

Publisher Cell Press

Specialties Biology
Biomedical Engineering
Science

Date 2021 Nov 11

PMID 34761231

Citations 1

Authors

Ashish A Kawale

Bjorn M Burmann

Affiliations

Soon will be listed here.

Abstract

The comprehensive delineation of inherent dynamic motions embedded in proteins, which can be crucial for their functional repertoire, is often essential yet remains poorly understood in the majority of cases. In this protocol, we outline detailed descriptions of the necessary steps for employing solution NMR spectroscopy for the in-depth amino acid level understanding of backbone dynamics of proteins. We describe the application of the protocol on the structurally analogous Tudor domains with disparate functionalities as a model system. For complete details on the use and execution of this protocol, please refer to Kawale and Burmann (2021).

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