The Creation of Unilateral Intermittent and Unintermittent Renal Ischemia-reperfusion Models in Rats

Overview

Journal Urol Ann

Specialty Urology

Date 2021 Nov 11

PMID 34759650

Authors

Mustafa Soytas

Duygu Gursoy

Mustafa Yucel Boz

Cagri Cakici

Ilknur Keskin

Turkan Yigitbasi

Selcuk Guven

Rahim Horuz

Selami Albayrak

Affiliations

Soon will be listed here.

Abstract

Background And Aim: This study aims to establish unilateral intermittent and unintermittent partial nephrectomy-like renal ischemia-reperfusion (I-R) model in rats and to compare the results with biochemical findings.

Material And Methods: The study was conducted on 24 adult 8-week-old male Wistar-Albino rats, each weighing s200-250 g. The rats were divided into three groups. In the Sham group ( = 8), the kidney was surgically exposed and closed. We designed experimental I-R models in the second group ( = 8, a total of 30-min ischemia model in the manner of 3 intermittent sets 8 minutes clamping and 2 min unclamping) and in the third group ( = 8, one session of 30-min unintermittent ischemia). In postoperative day 1, the rats were sacrificed, and the effects of I-R models on the renal tissue were comparatively assessed by evaluating serum Neutrophil Gelatinase-Associated Lipocalin (NGAL), serum kidney injury molecule-1 (KIM-1), urinary NGAL, urinary KIM-1, and serum creatinine levels.

Results: Urinary NGAL and KIM-1 levels were significantly higher in the continuous ischemia group when compared to those in the sham and intermittent ischemia groups ( < 0.05). In the intermittent ischemia group, urinary NGAL and urinary KIM-1 levels were significantly higher than those in the sham group ( < 0.05). Although the results of serum NGAL, serum KIM-1, and serum creatinine levels seemed to be in parallel to the results of urinary markers, no statistically significant difference was found.

Conclusion: Renal injury was significantly less in the intermittent I-R model when compared to that in the unintermittent I-R model in our experimental rat study.

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