Real-time Monitoring of Deformed Wing Virus-infected Bee Foraging Behavior Following Histone Deacetylase Inhibitor Treatment

Overview

Journal iScience

Publisher Cell Press

Date 2021 Nov 10

PMID 34755080

Citations 1

Authors

Cheng-Kang Tang

Yu-Hsien Lin

Joe-Air Jiang

Yun-Heng Lu

Chih-Hsuan Tsai

Yu-Chun Lin

Yun-Ru Chen

Carol-P Wu

Yueh-Lung Wu

Affiliations

Soon will be listed here.

Abstract

Impairment in the learning/memory behavior of bees is responsible for the massive disappearance of bee populations and its consequent agricultural economic losses. Such impairment might be because of o both pesticide exposure and pathogen infection, with a key contributor deformed wing virus (DWV). The present study found that sodium butyrate (NaB) significantly increased survival and reversed the learning/memory impairment of DWV-infected bees. A next-generation sequencing analysis showed that NaB affected the expression of genes involved in glycolytic processes and memory formation, which were suppressed by DWV infection. In addition, we performed a large-scale movement tracking experiment by using a wireless sensor network-based automatic real-time monitoring system and confirmed that NaB could improve the homing ability of DWV-infected bees. In short, we demonstrated the mechanism of how epigenetic regulation can resume the memory function of honeybees and suggest strategies for applying NaB to reduce the incidence of colony losses.

Citing Articles

Learning performance and GABAergic pathway link to deformed wing virus in the mushroom bodies of naturally infected honey bees.

Szymanski S, Baracchi D, Dingle L, Bowman A, Manfredini F J Exp Biol. 2024; 227(13).

PMID: 38894668 PMC: 11418184. DOI: 10.1242/jeb.246766.

References

Schroeder D, Martin S . Deformed wing virus: the main suspect in unexplained honeybee deaths worldwide. Virulence. 2012; 3(7):589-91. PMC: 3545936. DOI: 10.4161/viru.22219. View

Fuchikami M, Yamamoto S, Morinobu S, Okada S, Yamawaki Y, Yamawaki S . The potential use of histone deacetylase inhibitors in the treatment of depression. Prog Neuropsychopharmacol Biol Psychiatry. 2015; 64:320-4. DOI: 10.1016/j.pnpbp.2015.03.010. View

Shein N, Shohami E . Histone deacetylase inhibitors as therapeutic agents for acute central nervous system injuries. Mol Med. 2011; 17(5-6):448-56. PMC: 3105144. DOI: 10.2119/molmed.2011.00038. View

McKinsey T . Targeting inflammation in heart failure with histone deacetylase inhibitors. Mol Med. 2011; 17(5-6):434-41. PMC: 3105143. DOI: 10.2119/molmed.2011.00022. View

Vecsey C, Hawk J, Lattal K, Stein J, Fabian S, Attner M . Histone deacetylase inhibitors enhance memory and synaptic plasticity via CREB:CBP-dependent transcriptional activation. J Neurosci. 2007; 27(23):6128-40. PMC: 2925045. DOI: 10.1523/JNEUROSCI.0296-07.2007. View

Kajobe R, Marris G, Budge G, Laurenson L, Cordoni G, Jones B . First molecular detection of a viral pathogen in Ugandan honey bees. J Invertebr Pathol. 2010; 104(2):153-6. DOI: 10.1016/j.jip.2010.02.007. View

Zhou Y, Badgett M, Billard L, Bowen J, Orlando R, Willis J . Properties of the cuticular proteins of Anopheles gambiae as revealed by serial extraction of adults. PLoS One. 2017; 12(4):e0175423. PMC: 5395146. DOI: 10.1371/journal.pone.0175423. View

Garcez M, de Carvalho C, Mina F, Bellettini-Santos T, Schiavo G, Silva S . Sodium butyrate improves memory and modulates the activity of histone deacetylases in aged rats after the administration of d-galactose. Exp Gerontol. 2018; 113:209-217. DOI: 10.1016/j.exger.2018.10.005. View

Mordecai G, Brettell L, Pachori P, Villalobos E, Martin S, Jones I . Moku virus; a new Iflavirus found in wasps, honey bees and Varroa. Sci Rep. 2016; 6:34983. PMC: 5054524. DOI: 10.1038/srep34983. View

10.

Aizen M, Harder L . The global stock of domesticated honey bees is growing slower than agricultural demand for pollination. Curr Biol. 2009; 19(11):915-8. DOI: 10.1016/j.cub.2009.03.071. View

11.

Martin , Gunn . The transmission of deformed wing virus between honeybees (Apis mellifera L.) by the ectoparasitic mite varroa jacobsoni Oud . J Invertebr Pathol. 1999; 73(1):101-6. DOI: 10.1006/jipa.1998.4807. View

12.

Nicholls E, Botias C, Rotheray E, Whitehorn P, David A, Fowler R . Monitoring Neonicotinoid Exposure for Bees in Rural and Peri-urban Areas of the U.K. during the Transition from Pre- to Post-moratorium. Environ Sci Technol. 2018; 52(16):9391-9402. DOI: 10.1021/acs.est.7b06573. View

13.

Lin Y, Tai C, Broz V, Tang C, Chen P, Wu C . Adenosine Receptor Modulates Permissiveness of Baculovirus (Budded Virus) Infection via Regulation of Energy Metabolism in . Front Immunol. 2020; 11:763. PMC: 7198810. DOI: 10.3389/fimmu.2020.00763. View

14.

Wu C, Chang C, Wu J, Chiang M, Yang C, Chiang H . Mushroom body glycolysis is required for olfactory memory in Drosophila. Neurobiol Learn Mem. 2018; 150:13-19. DOI: 10.1016/j.nlm.2018.02.015. View

15.

Hu Y, Tang C, Wu C, Wu P, Yang E, Tai C . Histone deacetylase inhibitor treatment restores memory-related gene expression and learning ability in neonicotinoid-treated Apis mellifera. Insect Mol Biol. 2018; 27(4):512-521. DOI: 10.1111/imb.12390. View

16.

Marks P, Richon V, Rifkind R . Histone deacetylase inhibitors: inducers of differentiation or apoptosis of transformed cells. J Natl Cancer Inst. 2000; 92(15):1210-6. DOI: 10.1093/jnci/92.15.1210. View

17.

Genersch E, Yue C, Fries I, de Miranda J . Detection of Deformed wing virus, a honey bee viral pathogen, in bumble bees (Bombus terrestris and Bombus pascuorum) with wing deformities. J Invertebr Pathol. 2005; 91(1):61-3. DOI: 10.1016/j.jip.2005.10.002. View

18.

Yang X, Cox-Foster D . Impact of an ectoparasite on the immunity and pathology of an invertebrate: evidence for host immunosuppression and viral amplification. Proc Natl Acad Sci U S A. 2005; 102(21):7470-5. PMC: 1140434. DOI: 10.1073/pnas.0501860102. View

19.

Traniello I, Bukhari S, Kevill J, Ahmed A, Hamilton A, Naeger N . Meta-analysis of honey bee neurogenomic response links Deformed wing virus type A to precocious behavioral maturation. Sci Rep. 2020; 10(1):3101. PMC: 7033282. DOI: 10.1038/s41598-020-59808-4. View

20.

Chen Y, Evans J, Feldlaufer M . Horizontal and vertical transmission of viruses in the honey bee, Apis mellifera. J Invertebr Pathol. 2006; 92(3):152-9. DOI: 10.1016/j.jip.2006.03.010. View