Biochemical Characterization of Xylanase GH11 Isolated from Aspergillus Niger BCC14405 (XylB) and Its Application in Xylooligosaccharide Production

Overview

Journal Biotechnol Lett

Specialties Biochemistry
Biotechnology

Date 2021 Oct 31

PMID 34718907

Citations 1

Authors

Katesuda Aiewviriyasakul

Benjarat Bunterngsook

Hataikarn Lekakarn

Wipawee Sritusnee

Pattanop Kanokratana

Verawat Champreda

Affiliations

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Abstract

Objective: To develop an endo-β-1,4-xylanase with high specificity for production of prebiotic xylooligosaccharides that optimally works at moderate temperature desirable to reduce the energy cost in the production process.

Results: The xylB gene, encoding for a glycosyl hydrolase family 11 xylanase from a thermoresistant fungus, Aspergillus niger BCC14405 was expressed in a methylotrophic yeast P. pastoris KM71 in a secreted form. The recombinant XylB showed a high specific activity of 3852 and 169 U mg protein on beechwood xylan and arabinoxylan, respectively with no detectable side activities against different forms of cellulose (Avicel Ò PH101 microcrystalline cellulose, phosphoric acid swollen cellulose and carboxymethylcellulose). The enzyme worked optimally at 45 °C, pH 6.0. It showed a specific cleavage pattern by releasing xylobiose (X2) as the major product from xylooligosaccharides (X3 to X6) substrates. The highest XOS yield of 708 mg g substrate comprising X2, X3 and X6 was obtained from beechwood xylan hydrolysis.

Conclusion: The enzyme is potent for XOS production and for saccharification of lignocellulosic biomass.

Citing Articles

Biochemical characterization of a novel acidophilic β-xylanase from ND-1 and its synergistic hydrolysis of beechwood xylan.

Zheng F, Basit A, Zhuang H, Chen J, Zhang J, Chen W Front Microbiol. 2022; 13:998160.

PMID: 36199370 PMC: 9527580. DOI: 10.3389/fmicb.2022.998160.

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