Integrative Growth Physiology and Transcriptome Profiling of Probiotic KUB-AC5

Overview

Journal PeerJ

Specialties Biology
Environmental Health
General Medicine

Date 2021 Oct 28

PMID 34707932

Citations 2

Authors

Theeraphol Jatuponwiphat

Thanawat Namrak

Sunee Nitisinprasert

Massalin Nakphaichit

Wanwipa Vongsangnak

Affiliations

Soon will be listed here.

Abstract

KUB-AC5 has been widely used as probiotic in chicken for reduction. However, a preferable carbon source and growth phase is poorly characterized underlying metabolic responses on growth and inhibition effects of KUB-AC5. This study therefore aimed to investigate transcriptome profiling of KUB-AC5 revealing global metabolic responses when alteration of carbon sources and growth phases. Interestingly, KUB-AC5 grown under sucrose culture showed to be the best for fast growth and inhibition effects against Enteritidis S003 growth. Towards the transcriptome profiling and reporter proteins/metabolites analysis, the results showed that amino acid transport ABC systems as well as sucrose metabolism and transport are key metabolic responses at Logarithmic (L)-phase of KUB-AC5 growth. Considering the Stationary (S)-phase, we found the potential reporter proteins/metabolites involved in carbohydrate metabolism ., levansucrase and levan. Promisingly, levansucrase and levan were revealed to be candidates in relation to inhibition effects of KUB-AC5. Throughout this study, KUB-AC5 had a metabolic control in acclimatization to sucrose and energy pools through transcriptional co-regulation, which supported the cell growth and inhibition potentials. This study offers a perspective in optimizing fermentation condition through either genetic or physiological approaches for enhancing probiotic KUB-AC5 properties.

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Probing Genome-Scale Model Reveals Metabolic Capability and Essential Nutrients for Growth of Probiotic KUB-AC5.

Namrak T, Raethong N, Jatuponwiphat T, Nitisinprasert S, Vongsangnak W, Nakphaichit M Biology (Basel). 2022; 11(2).

PMID: 35205160 PMC: 8869380. DOI: 10.3390/biology11020294.

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