Green Biosynthesis, Characterization, and Cytotoxic Effect of Magnetic Iron Nanoparticles Using Brassica Oleracea Var Capitata Sub Var Rubra (red Cabbage) Aqueous Peel Extract

Overview

Journal Turk J Chem

Publisher Scientific and Technological Research Council of Turkey

Date 2021 Oct 28

PMID 34707435

Citations 3

Authors

Omer Erdogan

Salih Pasa

Gulen Melike Demirbolat

Ozge Cevik

Affiliations

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Abstract

The green method of nanoparticle synthesis, which is an environment and living-friendly method, is an updated subject that has appeared as an alternative to conventional methods such as physical and chemical synthesis. In this presented study, the green synthesis of magnetic iron oxide nanoparticles (IONPs) from iron (III) chloride by using aqueous peel extract has been reported. The prepared IONPs were characterized with fourier-transform infrared spectroscopy (FT-IR), ultraviolet-visible spectroscopy (UV-VIS), zeta potential, scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The cytotoxic effects of IONPs on MCF-7 breast cancer cell line were studied by MTT assay, and migrative effect of its were carried out by the wound healing assay. It was found that the mean particle size of IONPs was 675 ± 25 nm, and the polydispersity index was 0.265 PDI. It was also determined that these nanoparticles had an anti-proliferative impact on the MCF-7 breast cancer cell line depending on the dosage. Characterization results support the successful synthesis of nanoparticles, and the dose-dependent cytotoxic effects of nanoparticles on MCF-7 cells also make it a potential chemotherapeutic agent for breast cancer treatment.

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References

Marycz K, Sobierajska P, Roecken M, Kornicka-Garbowska K, Kepska M, Idczak R . Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action. J Nanobiotechnology. 2020; 18(1):33. PMC: 7027282. DOI: 10.1186/s12951-020-00590-w. View

Khan S, Musarrat J, Al-Khedhairy A . Countering drug resistance, infectious diseases, and sepsis using metal and metal oxides nanoparticles: Current status. Colloids Surf B Biointerfaces. 2016; 146:70-83. DOI: 10.1016/j.colsurfb.2016.05.046. View

Vangijzegem T, Stanicki D, Laurent S . Magnetic iron oxide nanoparticles for drug delivery: applications and characteristics. Expert Opin Drug Deliv. 2018; 16(1):69-78. DOI: 10.1080/17425247.2019.1554647. View

Huang L, Weng X, Chen Z, Megharaj M, Naidu R . Synthesis of iron-based nanoparticles using oolong tea extract for the degradation of malachite green. Spectrochim Acta A Mol Biomol Spectrosc. 2013; 117:801-4. DOI: 10.1016/j.saa.2013.09.054. View

Mahmoudi M, Simchi A, Imani M, Shokrgozar M, Milani A, Hafeli U . A new approach for the in vitro identification of the cytotoxicity of superparamagnetic iron oxide nanoparticles. Colloids Surf B Biointerfaces. 2009; 75(1):300-9. DOI: 10.1016/j.colsurfb.2009.08.044. View

Khan A, Gillis K, Clor J, Tyagarajan K . Simplified evaluation of apoptosis using the Muse cell analyzer. Postepy Biochem. 2013; 58(4):492-6. View

S S, K V, S P, N R, K K . In vitro cytotoxicity of zinc oxide, iron oxide and copper nanopowders prepared by green synthesis. Toxicol Rep. 2017; 4:427-430. PMC: 5615137. DOI: 10.1016/j.toxrep.2017.07.005. View

Saif S, Tahir A, Chen Y . Green Synthesis of Iron Nanoparticles and Their Environmental Applications and Implications. Nanomaterials (Basel). 2017; 6(11). PMC: 5245755. DOI: 10.3390/nano6110209. View

Pouliquen D, Le Jeune J, Perdrisot R, Ermias A, Jallet P . Iron oxide nanoparticles for use as an MRI contrast agent: pharmacokinetics and metabolism. Magn Reson Imaging. 1991; 9(3):275-83. DOI: 10.1016/0730-725x(91)90412-f. View

10.

Okoli C, Fornara A, Qin J, Toprak M, Dalhammar G, Muhammed M . Characterization of superparamagnetic iron oxide nanoparticles and its application in protein purification. J Nanosci Nanotechnol. 2012; 11(11):10201-6. DOI: 10.1166/jnn.2011.5007. View

11.

Saiyed Z, Ramchand C, Telang S . Isolation of genomic DNA using magnetic nanoparticles as a solid-phase support. J Phys Condens Matter. 2011; 20(20):204153. DOI: 10.1088/0953-8984/20/20/204153. View

12.

Senff-Ribeiro A, Echevarria A, Silva E, Franco C, Veiga S, Oliveira M . Cytotoxic effect of a new 1,3,4-thiadiazolium mesoionic compound (MI-D) on cell lines of human melanoma. Br J Cancer. 2004; 91(2):297-304. PMC: 2409799. DOI: 10.1038/sj.bjc.6601946. View

13.

Erdogan O, Abbak M, Demirbolat G, Birtekocak F, Aksel M, Pasa S . Green synthesis of silver nanoparticles via Cynara scolymus leaf extracts: The characterization, anticancer potential with photodynamic therapy in MCF7 cells. PLoS One. 2019; 14(6):e0216496. PMC: 6586393. DOI: 10.1371/journal.pone.0216496. View

14.

Shabnam N, Pardha-Saradhi P . Photosynthetic electron transport system promotes synthesis of Au-nanoparticles. PLoS One. 2013; 8(8):e71123. PMC: 3748038. DOI: 10.1371/journal.pone.0071123. View

15.

Mohanraj S, Kodhaiyolii S, Rengasamy M, Pugalenthi V . Green synthesized iron oxide nanoparticles effect on fermentative hydrogen production by Clostridium acetobutylicum. Appl Biochem Biotechnol. 2014; 173(1):318-31. DOI: 10.1007/s12010-014-0843-0. View

16.

Kettmann V, Kosfalova D, Jantova S, Cernakova M, Drimal J . In vitro cytotoxicity of berberine against HeLa and L1210 cancer cell lines. Pharmazie. 2004; 59(7):548-51. View

17.

Eslami S, Ebrahimzadeh M, Biparva P . Green synthesis of safe zero valent iron nanoparticles by leaf extract as an effective agent for reducing excessive iron in iron-overloaded mice, a thalassemia model. RSC Adv. 2022; 8(46):26144-26155. PMC: 9082760. DOI: 10.1039/c8ra04451a. View

18.

Gunalan S, Sivaraj R, Venckatesh R . Aloe barbadensis Miller mediated green synthesis of mono-disperse copper oxide nanoparticles: optical properties. Spectrochim Acta A Mol Biomol Spectrosc. 2012; 97:1140-4. DOI: 10.1016/j.saa.2012.07.096. View

19.

Bong Y, Shin W, Gautam M, Jeong Y, Lee A, Jang C . Determining the geographical origin of Chinese cabbages using multielement composition and strontium isotope ratio analyses. Food Chem. 2012; 135(4):2666-74. DOI: 10.1016/j.foodchem.2012.07.045. View

20.

Win K, Feng S . Effects of particle size and surface coating on cellular uptake of polymeric nanoparticles for oral delivery of anticancer drugs. Biomaterials. 2004; 26(15):2713-22. DOI: 10.1016/j.biomaterials.2004.07.050. View